LOCUS       Exported                 889 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  An improved chloride-conducting channelrhodopsin for light-induced 
            inhibition of neuronal activity in vivo. Cre inducible expression 
            (double floxed inversed ORF).
ACCESSION   .
VERSION     .
KEYWORDS    pAAV EF1a DIO iChloC 2A dsRed
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 889)
  TITLE     An improved chloride-conducting channelrhodopsin for light-induced 
            inhibition of neuronal activity in vivo. Cre inducible expression 
            (double floxed inverse ORF)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    34..67
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     protein_bind    118..151
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     complement(222..245)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(420..439)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     637..657
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     689..707
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      833..842
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     regulatory      837..846
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 gtgaggtacg gatcctctag agtcgactcc ggaataactt cgtataggat actttatacg
       61 aagttatgca gaatggtagc tggattgtag ctgctattag caatatgaaa cctcttaata
      121 acttcgtata gcatacatta tacgaagtta tggcgcgccc tacaggaaca ggtggtggcg
      181 gccctcggag cgctcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc
      241 cagcttggtg tccacgtagt agtagccggg cagctgcacg ggcttcttgg ccatgtagat
      301 ggtcttgaac tccaccaggt agtggccgcc gtccttcagc ttcagggcct ggtggatctc
      361 gcccttcagc acgccgtcgc gggggtacag gcgctcggtg gaggcctccc agcccatggt
      421 cttcttctgc attacggggc cgtcgggggg gaagttggtg ccgcggaact tcaccttgta
      481 gatcagcgtg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc
      541 gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttct tgtagtcggg
      601 gatgtcggcg gggtgcttca cgtacgcctt ggagccgtac tggaactggg gggacaggat
      661 gtcccaggcg aagggcaggg ggccgccctt ggtcaccttc agcttggcgg tctgggtgcc
      721 ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc
      781 ctccatgcgc accttgaagc gcatgaactc tttgatgacg tcctcggagg aggccaccat
      841 ggcggccgcg gatcccggtg cagggccggg attctcctcc acgtcaccg
//
