LOCUS       Exported                6301 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pGL3-p300 reporter
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Yu J, de Belle I, Liang H, Adamson ED
  TITLE     Coactivating factors p300 and CBP are transcriptionally 
            crossregulated by Egr1 in prostate cells, leading to divergent 
            responses.
  JOURNAL   Mol Cell. 2004 Jul 2. 15(1):83-94.
  PUBMED    15225550
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    238..359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(275..294)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     329..348
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(400..2052)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     1968..1986
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     regulatory      2049..2058
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    3630..3721
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(3662..3681)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    3735..3783
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3914..4369)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4051..4072)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4263..4282
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4396..4500
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4501..5361
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4719..4738)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5532..6120
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6021..6040
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6274..6291
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg
       61 acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc
      121 atcggtcgac ggatccttat cgattttacc acatttgtag aggttttact tgctttaaaa
      181 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
      241 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
      301 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
      361 catgtctgct cgaagcggcc ggccgccccg actctagaat tacacggcga tctttccgcc
      421 cttcttggcc tttatgagga tctctctgat ttttcttgcg tcgagttttc cggtaagacc
      481 tttcggtact tcgtccacaa acacaactcc tccgcgcaac tttttcgcgg ttgttacttg
      541 actggcgacg taatccacga tctctttttc cgtcatcgtc tttccgtgct ccaaaacaac
      601 aacggcggcg ggaagttcac cggcgtcatc gtcgggaaga cctgcgacac ctgcgtcgaa
      661 gatgttgggg tgttggagca agatggattc caattcagcg ggagccacct gatagccttt
      721 gtacttaatc agagacttca ggcggtcaac gatgaagaag tgttcgtctt cgtcccagta
      781 agctatgtct ccagaatgta gccatccatc cttgtcaatc aaggcgttgg tcgcttccgg
      841 attgtttaca taaccggaca taatcatagg acctctcaca cacagttcgc ctctttgatt
      901 aacgcccagc gttttcccgg tatccagatc cacaaccttc gcttcaaaaa atggaacaac
      961 tttaccgacc gcgcccggtt tatcatcccc ctcgggtgta atcagaatag ctgatgtagt
     1021 ctcagtgagc ccatatcctt gcctgatacc tggcagatgg aacctcttgg caaccgcttc
     1081 cccgacttcc ttagagaggg gagcgccacc agaagcaatt tcgtgtaaat tagataaatc
     1141 gtatttgtca atcagagtgc ttttggcgaa gaaggagaat agggttggca ccagcagcgc
     1201 actttgaatc ttgtaatcct gaaggctcct cagaaacagc tcttcttcaa atctatacat
     1261 taagacgact cgaaatccac atatcaaata tccgagtgta gtaaacattc caaaaccgtg
     1321 atggaatgga acaacactta aaatcgcagt atccggaatg atttgattgc caaaaatagg
     1381 atctctggca tgcgagaatc tcacgcaggc agttctatga ggcagagcga cacctttagg
     1441 cagaccagta gatccagagg agttcatgat cagtgcaatt gtcttgtccc tatcgaagga
     1501 ctctggcaca aaatcgtatt cattaaaacc gggaggtaga tgagatgtga cgaacgtgta
     1561 catcgactga aatccctggt aatccgtttt agaatccatg ataataattt tttggatgat
     1621 tgggagcttt ttttgcacgt tcaaaatttt ttgcaacccc tttttggaaa cgaacaccac
     1681 ggtaggctgc gaaatgccca tactgttgag caattcacgt tcattataaa tgtcgttcgc
     1741 gggcgcaact gcaactccga taaataacgc gcccaacacc ggcataaaga attgaagaga
     1801 gttttcactg catacgacga ttctgtgatt tgtattcagc ccatatcgtt tcatagcttc
     1861 tgccaaccga acggacattt cgaagtactc agcgtaagtg atgtccacct cgatatgtgc
     1921 atctgtaaaa gcaattgttc caggaaccag ggcgtatctc ttcatagcct tatgcagttg
     1981 ctctccagcg gttccatctt ccagcggata gaatggcgcc gggcctttct ttatgttttt
     2041 ggcgtcttcc atggtggctt taccaacagt accggaatgc caagcttcag gaaatctctt
     2101 cttcggcccg gggtccccgc gccgcaccca ggggtgggct ggggggctcc gacggcaccc
     2161 gagggggtgg gggaagttcc tttttctctt cgccgggtcg cagtggctgg agagggatgc
     2221 ggactcgata gaaaaggtaa gggcaagtgc gaggggccgg gcctgggccc aagcccggag
     2281 gagggcacaa gagcacaaat tcgccgagcg cgtccgctgc ggcgaattcc cgagaactcg
     2341 cggctctaga ggcgcagtcc ccggcccgcc acggccggcc cctgcccagc cgaggtctct
     2401 cggagctccg ccgccgccat caacctcttc ctcctcggcg ctgtcctcct ccttctcatc
     2461 gccacaagga ggcgggggcg aaaagggggg agaggaggag gcgacaagag acactcacct
     2521 cctcccggtg ccccctcccg ggcctgcgcc cccaccctcc gaaccctcct cctgccgccg
     2581 cgctccgcgc cgccgccgcc gcaccggccc ctaatggccg cagagaactc gcccgagccg
     2641 agagccaggc tggcaccgcc gcctcacatc gcgcggcggc cggcggaggc gcggcaagga
     2701 cgccgggagc ggagctggcc ggaccagagg gccgaggggg ccgggccgag cagcgccgcc
     2761 cgcacgcccg cgccgacgcg gagcccaccg aggaggcccc gctccggcgg cggcgcaggc
     2821 ggcccctcct cgcgccgcgc tcccctcctt cgccttcctt tcccgcggaa aataaataaa
     2881 tacggccgct gctacggccc ccaccgccgg cggccaggtg ctgggagcgc ccgccccccg
     2941 gggctccgtt cggggccgcc cgcggcggcg ggagaagacg ccgcggggcg cggggttatg
     3001 taatggtccc ccctaggcgt cgacgccggc ccggctgctg gaccgcggtt ggggtgtgtg
     3061 cggacgggag ggggctgggg gtcgcctcag cccgtgcgcc ccgggccccc tgccactgtc
     3121 tcggcccctg ccgcctctct ccgacccgac tcccctccgc actctctggc tcccgcagtt
     3181 agcactcagc ccggcgtcct gcttgcccac tctcgcccac tctctctcgc cctctctccc
     3241 ccccagccta gcacacaaac aagatggcgg ctgttgtttc ttccctctgc cgaatcctcc
     3301 ttacaggcta gcggtagcgg cggcggccgg aaatgagcca agggggggtg gaaaagggga
     3361 atggccgggc ctccggaggg gggcggggcc taggcccgag ccgctcacac aatgggggaa
     3421 gtggagcccg gggcggggcc tgtcccaatc acgggatccg ccctaggccc caacgcggtt
     3481 agcgcttccg caaactcccc cggcgtcacc ctgtggctcc ctttaaaggg gaggatttta
     3541 agagtgggga ggattttaag gggggagggg cgggggcgga ctcagaatct attttcagtc
     3601 ggtggatagt tacgagggta cctatcgata gagaaatgtt ctggcacctg cacttgcact
     3661 ggggacagcc tattttgcta gtttgttttg tttcgttttg ttttgatgga gagcgtatgt
     3721 tagtactatc gattcacaca aaaaaccaac acacagatgt aatgaaaata aagatatttt
     3781 attgcggccg ctccaagtac ctcccgtacc ttaatattac ttacttatca tggtagcttg
     3841 ggctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     3901 aatggcgaat ggcaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     3961 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     4021 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     4081 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     4141 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     4201 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     4261 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     4321 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     4381 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4441 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4501 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4561 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4621 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4681 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4741 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4801 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4861 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4921 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4981 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5041 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5101 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5161 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5221 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5281 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5341 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5401 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5461 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5521 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5581 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5641 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5701 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5761 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5821 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5881 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5941 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6001 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6061 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6121 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6181 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6241 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6301 g
//
