LOCUS Exported 732 bp ds-DNA linear SYN 23-MAY-2021 DEFINITION Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC). ACCESSION . VERSION . KEYWORDS pAAV Syn ChR E90R-D156N-T159C 2A tDimer SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 732) AUTHORS Wietek J, Wiegert JS, Adeishvili N, Schneider F, Watanabe H, Tsunoda SP, Vogt A, Elstner M, Oertner TG, Hegemann P TITLE Conversion of Channelrhodopsin into a Light-Gated Chloride Channel. JOURNAL Science. 2014 Mar 27. PUBMED 24674867 REFERENCE 2 (bases 1 to 732) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..732 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(114..137) /label=DsRed1-C /note="DsRed1, forward primer" primer_bind complement(312..331) /label=mCherry-F /note="mCherry, forward primer" primer_bind 529..549 /label=DsRed1-N /note="DsRed1, reverse primer" primer_bind 581..599 /label=mCherry-R /note="mCherry, reverse primer" ORIGIN 1 aattttgtaa tccagaggtt gattatcgat aagcttttgc tcgaagtcga cctacaggaa 61 caggtggtgg cggccctcgg agcgctcgta ctgttccacg atggtgtagt cctcgttgtg 121 ggaggtgatg tccagcttgg tgtccacgta gtagtagccg ggcagctgca cgggcttctt 181 ggccatgtag atggtcttga actccaccag gtagtggccg ccgtccttca gcttcagggc 241 ctggtggatc tcgcccttca gcacgccgtc gcgggggtac aggcgctcgg tggaggcctc 301 ccagcccatg gtcttcttct gcattacggg gccgtcgggg gggaagttgg tgccgcggaa 361 cttcaccttg tagatcagcg tgccgtcctg cagggaggag tcctgggtca cggtcaccac 421 gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt 481 cttgtagtcg gggatgtcgg cggggtgctt cacgtacgcc ttggagccgt actggaactg 541 gggggacagg atgtcccagg cgaagggcag ggggccgccc ttggtcacct tcagcttggc 601 ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc 661 gttcacggag ccctccatgc gcaccttgaa gcgcatgaac tctttgatga cgtcctcgga 721 ggaggcggtg cc //