LOCUS Exported 729 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION Fluorescent reporter for calcium signaling. ACCESSION . VERSION . KEYWORDS pZac2.1 gfaABC1D-lck-GCaMP6f SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 729) TITLE pZac2.1gfaABC1Dlck-GCaMP6f JOURNAL Unpublished REFERENCE 2 (bases 1 to 729) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..729 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 29..47 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 169..178 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 184..201 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 205..237 /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /label=T7 tag (gene 10 leader) /note="promotes efficient translation in E. coli" /translation="MASMTGGQQMG" CDS 241..264 /codon_start=1 /product="Xpress(TM) epitope tag, including an enterokinase recognition and cleavage site" /label=Xpress(TM) tag /translation="DLYDDDDK" regulatory 271..280 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 292..348 /codon_start=1 /product="calmodulin-binding peptide from avian smooth muscle myosin light chain kinase" /label=calmodulin-binding peptide /translation="RRKWNKTGHAVRAIGRLSS" primer_bind 561..582 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(688..709) /label=EGFP-N /note="EGFP, reverse primer" ORIGIN 1 caattacagc tcttaaggct agagtactta atacgactca ctataggcta gcctcgagaa 61 ttcaccatgg gctgtggctg cagctcaaac cctgaagatg actggatgga gaacattgac 121 gtgtgtgaga actgccatta tcccccgccg aagcttcgaa tggatccagc caccatgggt 181 tctcatcatc atcatcatca tggtatggct agcatgactg gtggacagca aatgggtcgg 241 gatctgtacg acgatgacga taaggatctc gccaccatgg tcgactcatc acgtcgtaag 301 tggaataaga caggtcacgc agtcagagct ataggtcggc tgagctcact cgagaacgtc 361 tatatcaagg ccgacaagca gaagaacggc atcaaggcga acttcaagat ccgccacaac 421 atcgaggacg gcggcgtgca gctcgcctac cactaccagc agaacacccc catcggcgac 481 ggccccgtgc tgctgcccga caaccactac ctgagcgtgc agtccaaact ttcgaaagac 541 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact 601 ctcggcatgg acgagctgta caagggcggt accggaggga gcatggtgag caagggcgag 661 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac 721 aagttcagc //