LOCUS Exported 6422 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBT140 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6422) AUTHORS Miyamichi K, Amat F, Moussavi F, Wang C, Wickersham I, Wall NR, Taniguchi H, Tasic B, Huang ZJ, He Z, Callaway EM, Horowitz MA, Luo L TITLE Cortical representations of olfactory input by trans-synaptic tracing. JOURNAL Nature. 2010 Dec 22. ():. PUBMED 21179085 REFERENCE 2 (bases 1 to 6422) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6422 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 923..943 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 947..1972 /codon_start=1 /product="site-specific recombinase" /label=Cre /note="Cre is a site-specific recombinase from bacteriophage P1. Recombination occurs at loxP sequences." /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG D" primer_bind complement(2016..2033) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2022..2246 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 2292..2720 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2379..2398) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2589..2610 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(2729..2749) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 2734..3063 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 2914..3049 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2976..2995 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3130..3924 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3184..3203) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3794..3813 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 4098..4219 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4135..4154) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4189..4208 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4268..4284) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4268..4284) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4281..4303) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4292..4308 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4316..4346) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4361..4382 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4499..4516) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4670..5255) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4750..4769) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5426..6286) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6049..6068 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6287..6391) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 901 taagcttggt accaccatgg gcccaaagaa gaagagaaag gtttcgaatt tactgaccgt 961 acaccaaaat ttgcctgcat taccggtcga tgcaacgagt gatgaggttc gcaagaacct 1021 gatggacatg ttcagggatc gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc 1081 cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac cggaaatggt ttcccgcaga 1141 acctgaagat gttcgcgatt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac 1201 tatccagcaa catttgggcc agctaaacat gcttcatcgt cggtccgggc tgccacgacc 1261 aagtgacagc aatgctgttt cactggttat gcggcggatc cgaaaagaaa acgttgatgc 1321 cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc 1381 actcatggaa aatagcgatc gctgccagga tatacgtaat ctggcatttc tggggattgc 1441 ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag atatctcacg 1501 tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc 1561 aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt 1621 ctctggtgta gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt 1681 tgccgcgcca tctgccacca gccagctatc aactcgcgcc ctggaaggga tttttgaagc 1741 aactcatcga ttgatttacg gcgctaagga tgactctggt cagagatacc tggcctggtc 1801 tggacacagt gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag tttcaatacc 1861 ggagatcatg caagctggtg gctggaccaa tgtaaatatt gtcatgaact atatccgtaa 1921 cctggatagt gaaacagggg caatggtgcg cctgctggaa gatggcgatt agcggccgct 1981 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca 2041 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 2101 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 2161 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 2221 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag 2281 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 2341 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 2401 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 2461 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 2521 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 2581 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 2641 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 2701 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 2761 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 2821 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 2881 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 2941 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 3001 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 3061 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga 3121 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag 3181 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc 3241 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg 3301 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc 3361 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg 3421 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct 3481 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg 3541 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat 3601 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc 3661 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg 3721 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc 3781 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct 3841 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat 3901 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga 3961 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct 4021 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg 4081 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata 4141 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 4201 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt 4261 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 4321 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 4381 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 4441 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 4501 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 4561 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 4621 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 4681 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 4741 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 4801 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 4861 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 4921 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 4981 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 5041 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 5101 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 5161 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca 5221 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 5281 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 5341 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 5401 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 5461 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 5521 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 5581 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 5641 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 5701 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 5761 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 5821 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 5881 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 5941 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 6001 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 6061 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 6121 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 6181 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 6241 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 6301 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 6361 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 6421 tc //