LOCUS Exported 9241 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS tetO-Hand2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9241) AUTHORS Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, Christoforou N, Epstein JA, Gearhart JD TITLE Optimization of direct fibroblast reprogramming to cardiomyocytes using calcium activity as a functional measure of success. JOURNAL J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 10.1016/j.yjmcc.2013.04.004. PUBMED 23591016 REFERENCE 2 (bases 1 to 9241) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9241 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2610..2628 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2652..2670 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2694..2712 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2736..2754 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2778..2796 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2820..2838 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" promoter 2871..2909 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2906..2930 /label=LNCX /note="Human CMV promoter, forward primer" primer_bind 3009..3028 /label=T7 /note="T7 promoter, forward primer" promoter 3009..3027 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 3059..3083 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(3765..3789) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 3815..3856 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" CDS 3866..3883 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" misc_feature 3933..4521 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3986..4006) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4404..4415) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(4524..4540) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4525..4541) /label=pBluescriptKS /note="For pBluescript vector" LTR 5046..5226 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(5252..5269) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5258..5482 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 5528..5956 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5615..5634) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5825..5846 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5965..5985) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5970..6299 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6150..6285 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6212..6231 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 6347..6394 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 6413..6787 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 6917..7038 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6954..6973) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7008..7027 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7087..7103) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7087..7103) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7100..7122) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7111..7127 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7135..7165) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7180..7201 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7318..7335) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7489..8077) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7569..7588) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8248..9108) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8871..8890 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9109..9213) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttatcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc 2641 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat 2701 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg 2761 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact 2821 ccctatcagt gatagagaaa agtgaaagtc gagctcggta cccgggtcga ggtaggcgtg 2881 tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac 2941 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc 3001 ccgaattgta atacgactca ctatagggag acccaagctg gctaggtaag cttgatcaac 3061 aagtttgtac aaaaaagcag aagggccgtc aaggcccacc atgagtctgg tggggggctt 3121 tccccaccac cccgtggtgc accatgaggg ctacccattc gccgcagccg ccgccgccgc 3181 tgccgccgcc gccgccagcc gctgcagcca cgaggagaac ccctacttcc acggctggct 3241 tattggccac ccggagatgt cgccccccga ctacagtatg gccctgtcct acagccccga 3301 gtacgccagc ggtgccgcgg gcctggacca ctcccattat gggggagtgc cgcccggcgc 3361 cgggcctccc ggcctggggg ggccgcgccc ggtgaagcgc cggggcaccg ccaaccgcaa 3421 ggagcggcgc aggactcaga gcatcaacag cgccttcgcc gagctgcgcg agtgcatccc 3481 caacgtgccc gccgacacca aactctccaa gatcaagaca ctgcgcctgg ccaccagcta 3541 catcgcctac ctcatggatc tgctggccaa ggacgaccag aacggagagg cggaggcctt 3601 caaggcggag atcaagaaga ccgacgtgaa agaggagaag aggaagaaag agctgaatga 3661 gatcttgaaa agcacagtga gcagcaacga caagaaaacc aaaggccgga caggctggcc 3721 acagcacgtc tgggccctgg agctcaagca gtagggcctc atgggcccag ctttcttgta 3781 caaagtggtt gatctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct 3841 cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacccgctg 3901 atcagcctca attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg 3961 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 4021 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 4081 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 4141 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 4201 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 4261 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 4321 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg 4381 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 4441 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 4501 ccctttgggc cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag 4561 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag 4621 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca 4681 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc 4741 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc 4801 ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt 4861 gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca 4921 cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag 4981 agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact 5041 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga 5101 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc 5161 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct 5221 ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca 5281 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc 5341 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg 5401 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct 5461 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg 5521 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 5581 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 5641 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 5701 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 5761 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt 5821 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 5881 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 5941 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 6001 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 6061 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 6121 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 6181 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg 6241 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 6301 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc 6361 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa 6421 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg 6481 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg 6541 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct 6601 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc 6661 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg 6721 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca 6781 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt 6841 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga 6901 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag 6961 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 7021 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta 7081 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 7141 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 7201 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 7261 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 7321 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 7381 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 7441 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 7501 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 7561 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 7621 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 7681 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 7741 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 7801 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 7861 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 7921 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 7981 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 8041 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 8101 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 8161 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 8221 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 8281 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 8341 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 8401 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 8461 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 8521 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 8581 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 8641 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 8701 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 8761 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 8821 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 8881 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 8941 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 9001 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 9061 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 9121 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 9181 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 9241 c //