LOCUS Exported 11660 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pLM-fSV2A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11660) AUTHORS Papapetrou EP, Lee G, Malani N, Setty M, Riviere I, Tirunagari LM, Kadota K, Roth SL, Giardina P, Viale A, Leslie C, Bushman FD, Studer L, Sadelain M TITLE Genomic safe harbors permit high I JOURNAL Nat Biotechnol. 2010 Dec 12. ():. PUBMED 21151124 REFERENCE 2 (bases 1 to 11660) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11660 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1091..1144 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 2564..2620 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 2621..3937 /codon_start=1 /product="human c-Myc proto-oncogene" /label=c-Myc /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA" CDS 3947..4006 /codon_start=1 /product="2A peptide from equine rhinitis A virus polyprotein" /label=E2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="QCTNYALLKLAGDVESNPGP" misc_feature 4967..5555 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5020..5040) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5438..5449) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" protein_bind 5688..5721 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." LTR 5748..5928 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" polyA_signal 6000..6121 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6037..6056) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6091..6110 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 6140..6275 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6202..6221 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(6307..6326) /label=T7 /note="T7 promoter, forward primer" promoter complement(6308..6326) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(6336..6353) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(6336..6352) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6345..6367) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 6497..6952 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6584..6603) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6794..6815 /label=F1ori-F /note="F1 origin, forward primer" promoter 6981..7085 /gene="bla" /label=AmpR promoter CDS 7086..7946 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7304..7323) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8117..8705 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8606..8625 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8859..8876 /label=L4440 /note="L4440 vector, forward primer" protein_bind 8993..9014 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9029..9059 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9067..9083 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9072..9094 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9091..9107 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9091..9107 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9126..9146 /label=T3 /note="T3 promoter, forward primer" promoter 9128..9146 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" LTR 9402..9582 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 9626..9751 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 10244..10477 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 10662..10706 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 10973..11090 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 11137..11647 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 11566..11584 /label=hPGK-F /note="Human PGK promoter, forward primer" ORIGIN 1 tatggcggga cacctggctt cggatttcgc cttctcgccc cctccaggtg gtggaggtga 61 tgggccaggg gggccggagc cgggctgggt tgatcctcgg acctggctaa gcttccaagg 121 ccctcctgga gggccaggaa tcgggccggg ggttgggcca ggctctgagg tgtgggggat 181 tcccccatgc cccccgccgt atgagttctg tggggggatg gcgtactgtg ggccccaggt 241 tggagtgggg ctagtgcccc aaggcggctt ggagacctct cagcctgagg gtgaagcagg 301 agtcggggtg gagagcaact ccgatggggc ctccccggag ccctgcaccg tcacccctgg 361 tgccgtgaag ctggagaagg agaagctgga gcaaaacccg gaggagtccc aggacatcaa 421 agctctgcag aaagaactcg agcaatttgc caagctcctg aagcagaaga ggatcaccct 481 gggatataca caggccgatg tggggctcac cctgggggtt ctatttggga aggtattcag 541 ccaaacgacc atctgccgct ttgaggctct gcagcttagc ttcaagaaca tgtgtaagct 601 gcggcccttg ctgcagaagt gggtggagga agctgacaac aatgaaaatc ttcaggagat 661 atgcaaagca gaaaccctcg tgcaggcccg aaagagaaag cgaaccagta tcgagaaccg 721 agtgagaggc aacctggaga atttgttcct gcagtgcccg aaacccacac tgcagcagat 781 cagccacatc gcccagcagc ttgggctcga gaaggatgtg gtccgagtgt ggttctgtaa 841 ccggcgccag aagggcaagc gatcaagcag cgactatgca caacgagagg attttgaggc 901 tgctgggtct cctttctcag ggggaccagt gtcctttcct ctggccccag ggccccattt 961 tggtacccca ggctatggga gccctcactt cactgcactg tactcctcgg tccctttccc 1021 tgagggggaa gcctttcccc ctgtctctgt caccactctg ggctctccca tgcattcaaa 1081 cggatctgga gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg 1141 ccccatggct gtcagcgacg cgctgctccc atctttctcc acgttcgcgt ctggcccggc 1201 gggaagggag aagacactgc gtcaagcagg tgccccgaat aaccgctggc gggaggagct 1261 ctcccacatg aagcgacttc ccccagtgct tcccggccgc ccctatgacc tggcggcggc 1321 gaccgtggcc acagacctgg agagcggcgg agccggtgcg gcttgcggcg gtagcaacct 1381 ggcgccccta cctcggagag agaccgagga gttcaacgat ctcctggacc tggactttat 1441 tctctccaat tcgctgaccc atcctccgga gtcagtggcc gccaccgtgt cctcgtcagc 1501 gtcagcctcc tcttcgtcgt cgccgtcgag cagcggccct gccagcgcgc cctccacctg 1561 cagcttcacc tatccgatcc gggccgggaa cgacccgggc gtggcgccgg gcggcacggg 1621 cggaggcctc ctctatggca gggagtccgc tccccctccg acggctccct tcaacctggc 1681 ggacatcaac gacgtgagcc cctcgggcgg cttcgtggcc gagctcctgc ggccagaatt 1741 ggacccggtg tacattccgc cgcagcagcc gcagccgcca ggtggcgggc tgatgggcaa 1801 gttcgtgctg aaggcgtcgc tgagcgcccc tggcagcgag tacggcagcc cgtcggtcat 1861 cagcgtcagc aaaggcagcc ctgacggcag ccacccggtg gtggtggcgc cctacaacgg 1921 cgggccgccg cgcacgtgcc ccaagatcaa gcaggaggcg gtctcttcgt gcacccactt 1981 gggcgctgga ccccctctca gcaatggcca ccggccggct gcacacgact tccccctggg 2041 gcggcagctc cccagcagga ctaccccgac cctgggtctt gaggaagtgc tgagcagcag 2101 ggactgtcac cctgccctgc cgcttcctcc cggcttccat ccccacccgg ggcccaatta 2161 cccatccttc ctgcccgatc agatgcagcc gcaagtcccg ccgctccatt accaagagct 2221 catgccaccc ggttcctgca tgccagagga gcccaagcca aagaggggaa gacgatcgtg 2281 gccccggaaa aggaccgcca cccacacttg tgattacgcg ggctgcggca aaacctacac 2341 aaagagttcc catctcaagg cacacctgcg aacccacaca ggtgagaaac cttaccactg 2401 tgactgggac ggctgtggat ggaaattcgc ccgctcagat gaactgacca ggcactaccg 2461 taaacacacg gggcaccgcc cgttccagtg ccaaaaatgc gaccgagcat tttccaggtc 2521 ggaccacctc gccttacaca tgaagaggca ttttggatct ggagcaacaa acttctcact 2581 actcaaacaa gcaggtgacg tggaggagaa tcccggccct atgcccctca acgttagctt 2641 caccaacagg aactatgacc tcgactacga ctcggtgcag ccgtatttct actgcgacga 2701 ggaggagaac ttctaccagc agcagcagca gagcgagctg cagcccccgg cgcccagcga 2761 ggatatctgg aagaaattcg agctgctgcc caccccgccc ctgtccccta gccgccgctc 2821 cgggctctgc tcgccctcct acgttgcggt cacacccttc tcccttcggg gagacaacga 2881 cggcggtggc gggagcttct ccacggccga ccagctggag atggtgaccg agctgctggg 2941 aggagacatg gtgaaccaga gtttcatctg cgacccggac gacgagacct tcatcaaaaa 3001 catcatcatc caggactgta tgtggagcgg cttctcggcc gccgccaagc tcgtctcaga 3061 gaagctggcc tcctaccagg ctgcgcgcaa agacagcggc agcccgaacc ccgcccgcgg 3121 ccacagcgtc tgctccacct ccagcttgta cctgcaggat ctgagcgccg ccgcctcaga 3181 gtgcatcgac ccctcggtgg tcttccccta ccctctcaac gacagcagct cgcccaagtc 3241 ctgcgcctcg caagactcca gcgccttctc tccgtcctcg gattctctgc tctcctcgac 3301 ggagtcctcc ccgcagggca gccccgagcc cctggtgctc catgaggaga caccgcccac 3361 caccagcagc gactctgagg aggaacaaga agatgaggaa gaaatcgatg ttgtttctgt 3421 ggaaaagagg caggctcctg gcaaaaggtc agagtctgga tcaccttctg ctggaggcca 3481 cagcaaacct cctcacagcc cactggtcct caagaggtgc cacgtctcca cacatcagca 3541 caactacgca gcgcctccct ccactcggaa ggactatcct gctgccaaga gggtcaagtt 3601 ggacagtgtc agagtcctga gacagatcag caacaaccga aaatgcacca gccccaggtc 3661 ctcggacacc gaggagaatg tcaagaggcg aacacacaac gtcttggagc gccagaggag 3721 gaacgagcta aaacggagct tttttgccct gcgtgaccag atcccggagt tggaaaacaa 3781 tgaaaaggcc cccaaggtag ttatccttaa aaaagccaca gcatacatcc tgtccgtcca 3841 agcagaggag caaaagctca tttctgaaga ggacttgttg cggaaacgac gagaacagtt 3901 gaaacacaaa cttgaacagc tacggaactc ttgtgcggga tctggacaat gtactaacta 3961 cgctttgttg aaactcgctg gcgatgttga aagtaacccc ggtcccatgt acaacatgat 4021 ggagacggag ctgaagccgc cgggcccgca gcaaacttcg gggggcggcg gcggcaactc 4081 caccgcggcg gcggccggcg gcaaccagaa aaacagcccg gaccgcgtca agcggcccat 4141 gaatgccttc atggtgtggt cccgcgggca gcggcgcaag atggcccagg agaaccccaa 4201 gatgcacaac tcggagatca gcaagcgcct gggcgccgag tggaaacttt tgtcggagac 4261 ggagaagcgg ccgttcatcg acgaggctaa gcggctgcga gcgctgcaca tgaaggagca 4321 cccggattat aaataccggc cccggcggaa aaccaagacg ctcatgaaga aggataagta 4381 cacgctgccc ggcgggctgc tggcccccgg cggcaatagc atggcgagcg gggtcggggt 4441 gggcgccggc ctgggcgcgg gcgtgaacca gcgcatggac agttacgcgc acatgaacgg 4501 ctggagcaac ggcagctaca gcatgatgca ggaccagctg ggctacccgc agcacccggg 4561 cctcaatgcg cacggcgcag cgcagatgca gcccatgcac cgctacgacg tgagcgccct 4621 gcagtacaac tccatgacca gctcgcagac ctacatgaac ggctcgccca cctacagcat 4681 gtcctactcg cagcagggca cccctggcat ggctcttggc tccatgggtt cggtggtcaa 4741 gtccgaggcc agctccagcc cccctgtggt tacctcttcc tcccactcca gggcgccctg 4801 ccaggccggg gacctccggg acatgatcag catgtatctc cccggcgccg aggtgccgga 4861 acccgccgcc cccagcagac ttcacatgtc ccagcactac cagagcggcc cggtgcccgg 4921 cacggccatt aacggcacac tgcccctctc acacatgtga gtcgacaatc aacctctgga 4981 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 5041 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 5101 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 5161 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 5221 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 5281 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 5341 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 5401 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 5461 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 5521 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta 5581 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 5641 ctggaagggc taattcactc ccaacgaaga caagatatcc tgctagcata acttcgtata 5701 gcatacatta tacgaagtta tgctagctcc ggatccggac ctgtactggg tctctctggt 5761 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 5821 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 5881 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat 5941 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga 6001 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 6061 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 6121 atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc 6181 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 6241 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcgtcg agacgtaccc 6301 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt 6361 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc 6421 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg 6481 aatggcgaat ggcgcgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 6541 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 6601 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 6661 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 6721 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 6781 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 6841 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 6901 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcccaggtg 6961 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 7021 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 7081 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 7141 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 7201 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 7261 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 7321 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 7381 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 7441 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 7501 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 7561 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 7621 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 7681 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 7741 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 7801 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 7861 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 7921 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 7981 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 8041 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 8101 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 8161 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 8221 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 8281 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 8341 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 8401 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 8461 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 8521 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 8581 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 8641 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 8701 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 8761 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 8821 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 8881 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 8941 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 9001 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 9061 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 9121 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc 9181 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca 9241 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt 9301 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg 9361 cagagatatt gtatttaagt gcctagctcg atacaataaa cgggtctctc tggttagacc 9421 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 9481 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 9541 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga 9601 cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg ctgaagcgcg 9661 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg 9721 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg 9781 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt 9841 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga 9901 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact 9961 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa 10021 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc 10081 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa 10141 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg 10201 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg 10261 ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg acggtacagg 10321 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg 10381 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc 10441 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa 10501 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac 10561 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct 10621 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat 10681 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt 10741 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg 10801 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc 10861 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag 10921 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa 10981 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag 11041 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg 11101 agactagcct cgagaagctt gatatcgaat tcccacgggg ttggggttgc gccttttcca 11161 aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag 11221 cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt 11281 cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg 11341 ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac 11401 cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc 11461 caatagcggc tgctcagcgg ggcgcgccga gagcagcggc cgggaagggg cggtgcggga 11521 ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg 11581 caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc 11641 tccccagggg gatccaccgg //