LOCUS       Exported                8517 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pLM-CMV-R-Cre
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8517)
  AUTHORS   Papapetrou EP, Lee G, Malani N, Setty M, Riviere I, Tirunagari LM, 
            Kadota K, Roth SL, Giardina P, Viale A, Leslie C, Bushman FD, Studer
            L, Sadelain M
  TITLE     Genomic safe harbors permit high I
  JOURNAL   Nat Biotechnol. 2010 Dec 12. ():.
  PUBMED    21151124
REFERENCE   2  (bases 1 to 8517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8517
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2..590
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(55..75)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(473..484)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             743..923
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    995..1116
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1032..1051)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1086..1105
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1135..1270
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1197..1216
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(1302..1321)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1303..1321)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1331..1348)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1331..1347)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1340..1362)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1492..1947
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1579..1598)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1789..1810
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1976..2080
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2081..2941
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2299..2318)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3112..3700
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3601..3620
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3854..3871
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3988..4009
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4024..4054
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4062..4078
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4067..4089
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4086..4102
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4086..4102
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4121..4141
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4123..4141
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             4397..4577
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4621..4746
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5239..5472
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5657..5701
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5968..6085
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6162..6465
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6466..6669
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6620..6640
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             6707..7414
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6852..6870)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7120..7139
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7424..7480
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7481..8512
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
ORIGIN
        1 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
       61 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
      121 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
      181 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
      241 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
      301 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
      361 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
      421 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
      481 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
      541 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg
      601 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
      661 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatcctgcta
      721 gctccggatc cggacctgta ctgggtctct ctggttagac cagatctgag cctgggagct
      781 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      841 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      901 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
      961 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     1021 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     1081 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     1141 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     1201 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     1261 tttggaggcc taggcttttg cgtcgagacg tacccaattc gccctatagt gagtcgtatt
     1321 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     1381 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     1441 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc gacgcgccct
     1501 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     1561 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     1621 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     1681 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     1741 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     1801 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     1861 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     1921 ttaacaaaat attaacgttt acaatttccc aggtggcact tttcggggaa atgtgcgcgg
     1981 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2041 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     2101 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     2161 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     2221 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     2281 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     2341 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     2401 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     2461 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     2521 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     2581 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     2641 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     2701 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     2761 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     2821 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     2881 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     2941 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     3001 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3061 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3121 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3181 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3241 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3301 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3361 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3421 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3481 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3541 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3601 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3661 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3721 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3781 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     3841 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     3901 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     3961 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     4021 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     4081 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     4141 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
     4201 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
     4261 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
     4321 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     4381 gctcgataca ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     4441 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     4501 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     4561 gtggaaaatc tctagcagtg gcgcccgaac agggacctga aagcgaaagg gaaaccagag
     4621 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     4681 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     4741 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     4801 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     4861 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     4921 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     4981 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     5041 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     5101 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     5161 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     5221 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     5281 tatgggcgca gcctcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     5341 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     5401 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     5461 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     5521 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     5581 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     5641 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     5701 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     5761 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     5821 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     5881 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     5941 gaacggatct cgacggtatc ggttaacttt taaaagaaaa ggggggattg gggggtacag
     6001 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     6061 acaaattaca aaaattcaaa attttatcga tcacgagact agcctcgata atagtaatca
     6121 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     6181 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     6241 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     6301 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     6361 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     6421 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     6481 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     6541 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     6601 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     6661 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtatgg tgagcaaggg
     6721 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     6781 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     6841 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     6901 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     6961 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     7021 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     7081 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     7141 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     7201 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     7261 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     7321 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     7381 ccactccacc ggcggcatgg acgagctgta caagggatct ggagcaacaa acttctcact
     7441 actcaaacaa gcaggtgacg tggaggagaa tcccggccct atgtccaatt tactgaccgt
     7501 acaccaaaat ttgcctgcat taccggtcga tgcaacgagt gatgaggttc gcaagaacct
     7561 gatggacatg ttcagggatc gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc
     7621 cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac cggaaatggt ttcccgcaga
     7681 acctgaagat gttcgcgatt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac
     7741 tatccagcaa catttgggcc agctaaacat gcttcatcgt cggtccgggc tgccacgacc
     7801 aagtgacagc aatgctgttt cactggttat gcggcggatc cgaaaagaaa acgttgatgc
     7861 cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc
     7921 actcatggaa aatagcgatc gctgccagga tatacgtaat ctggcatttc tggggattgc
     7981 ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag atatctcacg
     8041 tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc
     8101 aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt
     8161 ctctggtgta gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt
     8221 tgccgcgcca tctgccacca gccagctatc aactcgcgcc ctggaaggga tttttgaagc
     8281 aactcatcga ttgatttacg gcgctaagga tgactctggt cagagatacc tggcctggtc
     8341 tggacacagt gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag tttcaatacc
     8401 ggagatcatg caagctggtg gctggaccaa tgtaaatatt gtcatgaact atatccgtaa
     8461 cctggatagt gaaacagggg caatggtgcg cctgctggaa gatggcgatt aggtcga
//
