LOCUS Exported 8804 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAG305GPD-ccdB-EGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8804) TITLE S cerevisiae Advanced Gateway Destination Vectors REFERENCE 2 (bases 1 to 8804) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8804 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(29..51) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 151..170 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" CDS complement(664..1758) /codon_start=1 /gene="S. cerevisiae LEU2" /product="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" /label=LEU2 /note="yeast auxotrophic marker" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG DLGGSNSTTEVGDAVAEEVKKILA" promoter complement(1759..2166) /gene="S. cerevisiae LEU2" /label=LEU2 promoter rep_origin complement(2467..2922) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2604..2625) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2816..2835 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3048..3070 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3062..3079 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3063..3079 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 3086..3105 /label=T7 /note="T7 promoter, forward primer" promoter 3086..3104 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 3125..3768 /gene="S. cerevisiae TDH3" /label=GAP promoter /note="promoter for glyceraldehyde-3-phosphate dehydrogenase" primer_bind 3662..3685 /label=GPDpro-F /note="S. cerevisiae GPD promoter, forward primer" primer_bind 3774..3790 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 3774..3790 /label=pBluescriptSK /note="For pBluescript vector" protein_bind 3798..3922 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 3947..3977 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 4031..4690 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(4097..4116) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 5032..5337 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 5216..5235 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(5378..5502) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 5534..6253 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 5534..6250 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(5579..5600) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5840..5859) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6187..6208 /label=EGFP-C /note="EGFP, forward primer" terminator 6260..6507 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" primer_bind complement(6276..6294) /label=CYC1 /note="CYC1 transcription termination signal, reverse primer" promoter complement(6544..6562) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(6583..6599) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6583..6599) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6596..6618) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6607..6623 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6631..6661) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6676..6697 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6814..6831) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6985..7573) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7065..7084) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7744..8604) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8367..8386 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8605..8709) /gene="bla" /label=AmpR promoter primer_bind 8777..8795 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatcga ctacgtcgtt aaggccgttt ctgacagagt aaaattcttg agggaacttt 241 caccattatg ggaaatggtt caagaaggta ttgacttaaa ctccatcaaa tggtcaggtc 301 attgagtgtt ttttatttgt tgtatttttt tttttttaga gaaaatcctc caatatataa 361 attaggaatc atagtttcat gattttctgt tacacctaac tttttgtgtg gtgccctcct 421 ccttgtcaat attaatgtta aagtgcaatt ctttttcctt atcacgttga gccattagta 481 tcaatttgct tacctgtatt cctttacatc ctcctttttc tccttcttga taaatgtatg 541 tagattgcgt atatagtttc gtctacccta tgaacatatt ccattttgta atttcgtgtc 601 gtttctatta tgaatttcat ttataaagtt tatgtacaaa tatcataaaa aaagagaatc 661 tttttaagca aggattttct taacttcttc ggcgacagca tcaccgactt cggtggtact 721 gttggaacca cctaaatcac cagttctgat acctgcatcc aaaacctttt taactgcatc 781 ttcaatggcc ttaccttctt caggcaagtt caatgacaat ttcaacatca ttgcagcaga 841 caagatagtg gcgatagggt tgaccttatt ctttggcaaa tctggagcag aaccgtggca 901 tggttcgtac aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg acgcagatgg 961 caacaaaccc aaggaacctg ggataacgga ggcttcatcg gagatgatat caccaaacat 1021 gttgctggtg attataatac catttaggtg ggttgggttc ttaactagga tcatggcggc 1081 agaatcaatc aattgatgtt gaaccttcaa tgtagggaat tcgttcttga tggtttcctc 1141 cacagttttt ctccataatc ttgaagaggc caaaacatta gctttatcca aggaccaaat 1201 aggcaatggt ggctcatgtt gtagggccat gaaagcggcc attcttgtga ttctttgcac 1261 ttctggaacg gtgtattgtt cactatccca agcgacacca tcaccatcgt cttcctttct 1321 cttaccaaag taaatacctc ccactaattc tctgacaaca acgaagtcag tacctttagc 1381 aaattgtggc ttgattggag ataagtctaa aagagagtcg gatgcaaagt tacatggtct 1441 taagttggcg tacaattgaa gttctttacg gatttttagt aaaccttgtt caggtctaac 1501 actaccggta ccccatttag gaccacccac agcacctaac aaaacggcat cagccttctt 1561 ggaggcttcc agcgcctcat ctggaagtgg aacacctgta gcatcgatag cagcaccacc 1621 aattaaatga ttttcgaaat cgaacttgac attggaacga acatcagaaa tagctttaag 1681 aaccttaatg gcttcggctg tgatttcttg accaacgtgg tcacctggca aaacgacgat 1741 cttcttaggg gcagacatta gaatggtata tccttgaaat atatatatat atattgctga 1801 aatgtaaaag gtaagaaaag ttagaaagta agacgattgc taaccaccta ttggaaaaaa 1861 caataggtcc ttaaataata ttgtcaactt caagtattgt gatgcaagca tttagtcatg 1921 aacgcttctc tattctatat gaaaagccgg ttccggcgct ctcacctttc ctttttctcc 1981 caatttttca gttgaaaaag gtatatgcgt caggcgacct ctgaaattaa caaaaaattt 2041 ccagtcatcg aatttgattc tgtgcgatag cgcccctgtg tgttctcgtt atgttgagga 2101 aaaaaataat ggttgctaag agattcgaac tcttgcatct tacgatacct gagtattccc 2161 acagttaact gcggtcaaga tatttcttga atcaggcgcc ttagaccgct cggccaaaca 2221 accaattact tgttgagaaa tagagtataa ttatcctata aatataacgt ttttgaacac 2281 acatgaacaa ggaagtacag gacaattgat tttgaagaga atgtggattt tgatgtaatt 2341 gttgggattc catttttaat aaggcaataa tattaggtat gtagatatac tagaagttct 2401 cctcgaccgg tcgatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 2461 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 2521 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 2581 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 2641 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 2701 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 2761 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 2821 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 2881 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct 2941 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 3001 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 3061 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcag 3121 tttatcatta tcaatactcg ccatttcaaa gaatacgtaa ataattaata gtagtgattt 3181 tcctaacttt atttagtcaa aaaattagcc ttttaattct gctgtaaccc gtacatgccc 3241 aaaatagggg gcgggttaca cagaatatat aacatcgtag gtgtctgggt gaacagttta 3301 ttcctggcat ccactaaata taatggagcc cgctttttaa gctggcatcc agaaaaaaaa 3361 agaatcccag caccaaaata ttgttttctt caccaaccat cagttcatag gtccattctc 3421 ttagcgcaac tacagagaac aggggcacaa acaggcaaaa aacgggcaca acctcaatgg 3481 agtgatgcaa cctgcctgga gtaaatgatg acacaaggca attgacccac gcatgtatct 3541 atctcatttt cttacacctt ctattacctt ctgctctctc tgatttggaa aaagctgaaa 3601 aaaaaggttg aaaccagttc cctgaaatta ttcccctact tgactaataa gtatataaag 3661 acggtaggta ttgattgtaa ttctgtaaat ctatttctta aacttcttaa attctacttt 3721 tatagttagt ctttttttta gttttaaaac accagaactt agtttcgacg gattctagaa 3781 ctagtggatc ccccatcaca agtttgtaca aaaaagctga acgagaaacg taaaatgata 3841 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa 3901 cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc 3961 ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa 4021 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca 4081 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt 4141 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc 4201 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat 4261 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga 4321 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct 4381 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg 4441 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga 4501 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta 4561 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga 4621 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg 4681 cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg 4741 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag 4801 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc 4861 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc 4921 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc 4981 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt 5041 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat 5101 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca 5161 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg 5221 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc 5281 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg 5341 tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt 5401 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat 5461 atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatgggct gcaggaattc 5521 gatatcaagc ttaatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 5581 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 5641 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 5701 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 5761 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 5821 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 5881 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 5941 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 6001 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 6061 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 6121 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 6181 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 6241 gctgtacaag taactcgagt catgtaatta gttatgtcac gcttacattc acgccctccc 6301 cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt 6361 tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt 6421 tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt 6481 ttgggacgct cgaaggcttt aatttgcggc cgtcgagggg gggcccggta ccagcttttg 6541 ttccctttag tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt 6601 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 6661 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 6721 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 6781 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 6841 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 6901 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 6961 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 7021 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 7081 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 7141 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 7201 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 7261 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 7321 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 7381 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 7441 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 7501 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 7561 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 7621 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 7681 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 7741 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 7801 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 7861 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 7921 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 7981 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 8041 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 8101 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 8161 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 8221 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 8281 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 8341 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 8401 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 8461 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 8521 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 8581 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 8641 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 8701 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 8761 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc //