LOCUS Exported 12244 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS EF.hHES1.Ubc.GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12244) AUTHORS Yu X, Alder JK, Chun JH, Friedman AD, Heimfeld S, Cheng L, Civin CI TITLE HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding. JOURNAL Stem Cells. 2006 Apr . 24(4):876-88. PUBMED 16513761 REFERENCE 2 (bases 1 to 12244) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12244 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" primer_bind 2624..2640 /label=KS primer /note="common sequencing primer, one of multiple similar variants" promoter 2731..3909 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2962..3900 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3857..3877 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" primer_bind 3926..3945 /label=T7 /note="T7 promoter, forward primer" promoter 3926..3944 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 4912..6122 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 5959..5978 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" regulatory 6170..6179 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 6176..6895 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 6176..6892 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6221..6242) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6482..6501) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6829..6850 /label=EGFP-C /note="EGFP, forward primer" misc_feature 6936..7524 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6989..7009) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7407..7418) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(7527..7543) /label=KS primer /note="common sequencing primer, one of multiple similar variants" LTR 8049..8229 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(8255..8272) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 8261..8485 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 8531..8959 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8618..8637) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8828..8849 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(8968..8988) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 8973..9302 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 9153..9288 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9215..9234 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 9350..9397 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 9416..9790 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 9920..10041 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9957..9976) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10011..10030 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(10090..10106) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10090..10106) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(10103..10125) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 10114..10130 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(10138..10168) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10183..10204 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(10321..10338) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10492..11080) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10572..10591) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(11251..12111) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11874..11893 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(12112..12216) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta agtttaaacg ggccctctag actcgaggtc gacggtatcg 2641 ataagctttg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac 2701 cttctaggtc ttgaaaggag tgcctcgtga ggctccggtg cccgtcagtg ggcagagcgc 2761 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag 2821 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc 2881 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac 2941 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt 3001 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt 3061 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc 3121 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc 3181 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt 3241 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat 3301 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag 3361 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag 3421 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc 3481 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc 3541 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag 3601 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg 3661 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct 3721 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact 3781 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt 3841 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag 3901 gtgtcgtgag gaattagctt ggtactaata cgactcacta tagggagacc caagctggct 3961 agttaagctt gatctagaga taaaaaaaaa aaaaaaagga aaatgccagc tgatataatg 4021 gagaaaaatt cctcgtcccc ggtggctgct accccagcca gtgtcaacac gacaccggat 4081 aaaccaaaga cagcatctga gcacagaaag tcatcaaagc ctattatgga gaaaagacga 4141 agagcaagaa taaatgaaag tctgagccag ctgaaaacac tgattttgga tgctctgaag 4201 aaagatagct cgcggcattc caagctggag aaggcggaca ttctggaaat gacagtgaag 4261 cacctccgga acctgcagcg ggcgcagatg acggctgcgc tgagcacaga cccaagtgtg 4321 ctggggaagt accgagccgg cttcagcgag tgcatgaacg aggtgacccg cttcctgtcc 4381 acgtgcgagg gcgttaatac cgaggtgcgc actcggctgc tcggccacct ggccaactgc 4441 atgacccaga tcaatgccat gacctacccc gggcagccgc accccgcctt gcaggcgccg 4501 ccaccgcccc caccgggacc cggcggcccc cagcacgcgc cgttcgcgcc gccgccgcca 4561 ctcgtgccca tccccggggg cgcggcgccc cctcccggcg gcgccccctg caagctgggc 4621 agccaggctg gagaggcggc taaggtgttt ggaggcttcc aggtggtacc ggctcccgat 4681 ggccagtttg ctttcctcat tcccaacggg gccttcgcgc acagcggccc tgtcatcccc 4741 gtctacacca gcaacagcgg cacctccgtg ggccccaacg cagtgtcacc ttccagcggc 4801 ccctcgctta cggcggactc catgtggagg ccgtggcgga actgaggggg ctatcgaatt 4861 cccaccacac tggactagtg gatccggggg taccgcgtcg aggggtgcag cggcctccgc 4921 gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag 4981 acgaagggcg caggagcgtt cctgatcctt ccgcccggac gctcaggaca gcggcccgct 5041 gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact 5101 tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt 5161 cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata 5221 aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct 5281 tgtttgtgga tcgctgtgat cgtcacttgg tgagttgcgg gctgctgggc tggccggggc 5341 tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg 5401 tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcacaaaatg 5461 gcggctgttc ccgagtcttg aatggaagac gcttgtaagg cgggctgtga ggtcgttgaa 5521 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg 5581 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt 5641 ttgtcactga ctggagaact cgggtttgtc gtctggttgc gggggcggca gttatgcggt 5701 gccgttgggc agtgcacccg tacctttggg agcgcgcgcc tcgtcgtgtc gtgacgtcac 5761 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt 5821 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc 5881 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta 5941 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg 6001 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt 6061 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag 6121 acgaagcttg ggctgcaggt cgactctaga ggatccccgg gtaccggtcg ccaccatggt 6181 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 6241 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 6301 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 6361 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 6421 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 6481 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 6541 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 6601 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 6661 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 6721 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 6781 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 6841 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg 6901 ccgcgactct agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt 6961 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 7021 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 7081 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 7141 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 7201 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 7261 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 7321 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc 7381 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 7441 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 7501 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg 7561 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac 7621 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt 7681 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 7741 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact 7801 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat 7861 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga 7921 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat 7981 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg 8041 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 8101 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 8161 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 8221 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag 8281 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact 8341 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt 8401 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat 8461 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg 8521 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 8581 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 8641 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 8701 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 8761 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 8821 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 8881 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 8941 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc 9001 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 9061 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 9121 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 9181 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 9241 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 9301 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta 9361 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc 9421 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt 9481 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt 9541 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac 9601 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt 9661 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg 9721 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga 9781 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg 9841 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct 9901 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa 9961 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 10021 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc 10081 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 10141 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 10201 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 10261 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 10321 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 10381 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 10441 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 10501 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 10561 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 10621 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 10681 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 10741 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 10801 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 10861 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 10921 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 10981 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 11041 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 11101 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 11161 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 11221 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 11281 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 11341 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 11401 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 11461 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 11521 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 11581 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 11641 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 11701 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 11761 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 11821 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 11881 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 11941 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 12001 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 12061 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 12121 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 12181 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 12241 tgac //