LOCUS       Exported                2958 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pUT1111-HQ858674
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2958)
  TITLE     Maize TF ORFeome collection
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(515..614)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(631..650)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(632..650)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(655..671)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(655..671)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(773..792)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             784..1593
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(851..870)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1740..2328
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2229..2248
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2482..2499
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      2658..2685
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      2777..2863
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2912..2934
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2926..2943
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2927..2943
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atgggctccg ccgatcgctg taagatcgac gggatcgtgg cggcggagaa agccaccagg
      181 agctgcgtcg aatgccgcgc caccaccacg cccatgtggc gcagcggccc gaccgggccc
      241 aggtcacttt gcaacgcctg tgggatccgg taccgaaaaa agaggaggca agagcttggc
      301 ctagaccgca agctgcagca acaacaaaat aatggcgagg cgaagacgga tgaagcaaaa
      361 gacagctcta gcaacagcag cagcgggagc agcaacttgc aggtggtgca gaaacggagg
      421 ctgctaatgg gagtggagga ggctgctctg ctgctgatga ccctatcttc ttcgcccaca
      481 tccacgttgc ttcatggaaa gggtgggcgc gccgacccag ctttcttgta caaagttggc
      541 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
      601 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
      661 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
      721 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
      781 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
      841 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
      901 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
      961 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     1021 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     1081 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     1141 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     1201 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     1261 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     1321 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     1381 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     1441 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     1501 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     1561 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     1621 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     1681 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1741 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1801 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1861 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1921 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1981 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2041 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2101 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2161 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2221 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2281 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2341 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2401 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2461 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     2521 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     2581 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     2641 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     2701 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     2761 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     2821 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     2881 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     2941 agtcttaagc tcgggccc
//
