LOCUS Exported 4125 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1136-HQ858680 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4125) TITLE Maize TF ORFeome collection REFERENCE 2 (bases 1 to 4125) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4125 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1682..1781) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1798..1817) /label=T7 /note="T7 promoter, forward primer" promoter complement(1799..1817) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1822..1838) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1822..1838) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1940..1959) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1951..2760 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2018..2037) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2907..3495 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3396..3415 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3649..3666 /label=L4440 /note="L4440 vector, forward primer" terminator 3825..3852 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3944..4030 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 4079..4101 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 4093..4110 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 4094..4110 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc 121 atggagctcg ccggagcagc ctcccctcac ccgccgccgg aatcccacgt ggcgccgccc 181 cgcccacctc cgcagccgcc ggaagaggat ttatgtgaag atagagggga catgagtgtc 241 accggcgaaa agccatgcac acatcgggaa tcagatgttg gtcagacaaa tagttgtagc 301 cttaacaatt ccagtgagtg tgagaatcat acacccagca acgacgaaat atcggaacca 361 gagtccaatt tggagatggc caagactgat cagggcggcg atgtgccgag cggagagaag 421 gtcctgaaga agccagacaa gatcctgccg tgccctcgct gcaacagcat ggacacgaag 481 ttctgctact acaacaacta caacatcaag cagccaaggc atttctgcaa gagctgtcag 541 aggtactgga ccgcaggcgg aagcatgaga aacatccccg ttggtgctgg gaggcgcaag 601 agcaagagct ccagcgcgag ttgccgcagc gtgttgattc ccgttccagg cagcagtagt 661 gtagctaatc ctggcggaga ggcttccctg tttccgttgt ccgtaaaagc aaaccaagca 721 gcagttagct tcgggcctga ttcccccctc tgcacctcca tggcctccgt gctgaagatc 781 ggaggaggag gagagcaggt taagagctgc agccctgcct cagcagcagc acagcccagg 841 aacggagaaa cccagaccca gacgtgcgca ccttcttctg ctgctacaac accatcagat 901 ggtccaggga atggattgca gaaaggagca gagagcgcac accaaaacca aaaccaaaac 961 caaaacggaa tcattgggca cagcaacgga gtcactccag tgcatcctat accgttcttc 1021 cccggaccgc ctttcgtgta cccctggagt ccagcatgga acggcattcc cgccatggca 1081 gcggcggtgt gcgcagcccc agcgacagcc gaagcagcga tttcatcaga acacggcacc 1141 gcgagcagca ccgtccagtg gaacgtgcca ccagcgatcg tgcccgtgct gccaccggga 1201 ttctgcggcc cgatcccagt cccggtaatc ccgccgcctt ccgtctggcc actgatcact 1261 ccctggccca acgcagcatg gagcgcgccg tggctcgggc ctagcgctag cgtgccgccg 1321 gggtcatctc ggagcagcgg cagcagcacg tgctccgaca gcggctgcgg ctccggctcc 1381 cccgtcctgg gaaagcactc gagggagtcg aggccgcagg gcgacgagaa ggcggaacga 1441 cggtgcctgt ggatccccaa gacgctccgg atcgacgacc ctgtcgaggc cgccaagagc 1501 tcgatctgga cgacgctcgg gatcgagcct ggcgaccggg gcatgttcag gccgttccag 1561 tcgaagcatg gacggcagca ggagctgcaa gcgtccggcg ccgctcgcgc cctgcaggcc 1621 aacccggcgg ctctgtcgcg ctcgcagtct tttcaggaga cgacgaaggg tgggcgcgcc 1681 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc 1741 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc 1801 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa 1861 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct 1921 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg 1981 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat 2041 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg 2101 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta 2161 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat 2221 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa 2281 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat 2341 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg 2401 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc 2461 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca 2521 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata 2581 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta 2641 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt 2701 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa 2761 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc 2821 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac 2881 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 2941 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 3001 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 3061 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 3121 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 3181 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 3241 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat 3301 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 3361 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 3421 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 3481 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 3541 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 3601 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 3661 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 3721 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 3781 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca 3841 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc 3901 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga 3961 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc 4021 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc 4081 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc //