LOCUS Exported 821 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION Express sgRNA and dCas9-KRAB from lentiviral vector. ACCESSION . VERSION . KEYWORDS pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 821) AUTHORS Thakore PI, D'Ippolito AM, Song L, Safi A, Shivakumar NK, Kabadi AM, Reddy TE, Crawford GE, Gersbach CA TITLE Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements. JOURNAL Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub 2015 Oct 26. PUBMED 26501517 REFERENCE 2 (bases 1 to 821) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..821 /organism="synthetic DNA construct" /mol_type="other DNA" misc_RNA complement(32..107) /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" promoter complement(132..372) /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind complement(182..201) /label=LKO.1 5' /note="Human U6 promoter, forward primer" primer_bind complement(352..372) /label=hU6-F /note="Human U6 promoter, forward primer" promoter 405..804 /label=UbC promoter /note="human ubiquitin C promoter" ORIGIN 1 gcatacatta tacgaagtta ttaaaaaaaa agcaccgact cggtgccact ttttcaagtt 61 gataacggac tagccttatt ttaacttgct atttctagct ctaaaactga gacgtccgtc 121 tccggtgttt cgtcctttcc acaagatata taaagccaag aaatcgaaat actttcaagt 181 tacggtaagc atatgatagt ccattttaaa acataatttt aaaactgcaa actacccaag 241 aaattattac tttctacgtc acgtattttg tactaatatc tttgtgttta cagtcaaatt 301 aattccaatt atctctctaa cagccttgta tcgtatatgc aaatatgaag gaatcatggg 361 aaataggccc tcttaattaa ttaacccgtg tcggctccag atctggcctc cgcgccgggt 421 tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg 481 gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata 541 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga 601 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct 661 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc 721 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt 781 ggatcgctgt gatcgtcact tggtgagtag cgggctgctg g //