LOCUS Exported 3708 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1141-HQ858685 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3708) TITLE Maize TF ORFeome collection REFERENCE 2 (bases 1 to 3708) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3708 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1265..1364) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1381..1400) /label=T7 /note="T7 promoter, forward primer" promoter complement(1382..1400) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1405..1421) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1405..1421) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1523..1542) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1534..2343 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1601..1620) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2490..3078 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2979..2998 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3232..3249 /label=L4440 /note="L4440 vector, forward primer" terminator 3408..3435 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3527..3613 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3662..3684 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3676..3693 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3677..3693 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc 121 atgacgccgg cggcgtcgcc cgcgcagttc gcgccggcgc ccctcaggat ggggtacggg 181 cggccggcgc ccgtgatggg catgtggagc agcgagccgt tcaaggtcga cagcggcggg 241 cacgccacct gcagcgcgtc caccgtcatg gaggcggaca ccaagctcga gacaagcagg 301 ctacaagatg ttccacaggt agcactcgag ccagagagga gtacggatca ggaaacgagc 361 aggccgccag agagggtcat gagaaggctc gcgcagaaca gggaggctgc tcggaagagt 421 cgcctgcgga aaaaggccta catccagcag ctagagacaa gccggatgaa gctggcccag 481 ctagagctgg agcttcaacg ggctcgacgg cagcagggcg cgtacgcaaa tgggagcatg 541 ggagacccag ctctcggata cacagggtcg atcgatccag gcgtcgccgc gttcgagatc 601 gaatacaggc actgggtgga cgagcagaag cggcacacgg cggagctgat gtcggcgctc 661 caggggcagc agacgtcgga gctggagctc cgcttgctgg tggagacggg gctcagcaac 721 tacgagcacc tcttcaggat caaggcgctg gccgccaacg cggacgtgtt ccacgtcatg 781 tcgggcgtgt ggaagacccc cgccgagagg ttcttcctct ggatcggcgg gttcaggccg 841 tcggaggtcc taaagattct gagcccgcag ctggagccgc tggcggaggc gcagcgcatg 901 ctcgtgggcg gcctccagca cacgtcaacg caggcggagg acgcgctgtc gcagggcatg 961 gagaagctac agcagaacct cgccgagatc ctgaccgccg aggctgaccc cttcggggcc 1021 cccgacgcct acatgctgca gatggccact gcggtggaga agctgaaaga gctcgtcaac 1081 ttcgtcaccc aggcggacca tctccggctg atgacgctgc aacagatgca caagatcctg 1141 acgacgcggc aggcggccag gggcctgctg gcgctcggcg actacttcca gcgcctccgc 1201 acgctgagct cgctgtgggc ggcgcgcccg cgggaggcgg cgattagcaa gggtgggcgc 1261 gccgacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt 1321 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc 1381 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct 1441 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg 1501 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg 1561 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat 1621 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag 1681 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga 1741 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct 1801 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa 1861 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg 1921 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag 1981 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat 2041 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat 2101 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta 2161 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc 2221 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat 2281 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc 2341 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac 2401 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca 2461 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 2521 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 2581 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt 2641 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 2701 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 2761 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 2821 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 2881 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 2941 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 3001 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 3061 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 3121 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 3181 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 3241 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 3301 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 3361 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg 3421 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc 3481 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca 3541 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct 3601 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt 3661 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggccc //