LOCUS Exported 3255 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1144-HQ858688 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3255) TITLE Maize TF ORFeome collection REFERENCE 2 (bases 1 to 3255) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3255 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(812..911) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(928..947) /label=T7 /note="T7 promoter, forward primer" promoter complement(929..947) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(952..968) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(952..968) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1070..1089) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1081..1890 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1148..1167) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2037..2625 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2526..2545 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2779..2796 /label=L4440 /note="L4440 vector, forward primer" terminator 2955..2982 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3074..3160 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3209..3231 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3223..3240 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3224..3240 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc 121 atgggaggag gagccaccaa cctacctccg ggcttccatt tcttcccttc agacgaagac 181 ctcgtcgtcc atttcctccg ccgcaaagcc gccaacctcc cgtgccgccc cgacatcgtc 241 ccgacgatcc tccttcaccg ctacgacccg tgggagctga acggcacggc gctgcaggct 301 gggaaccagt ggtacttctt cagtagccac gcccacgccc acgcccactc cgcggcgggg 361 cagaccagaa cctctccgtc cgggtactgg aaccccgccg gcgccgatga aacagtagta 421 ctagcgagca gcggccgtgt cgttggcctc aagaagacgc tcgtcttctg caccggagag 481 cctttcagag ggttcagaac gaactgggtc atgcatgagt accaccttca ggacgggccg 541 gccgggtgca atgtcggcgg gagcagcggc gccaccaccc caagtagctc cagcagctcc 601 ggcagcagca ggagatcgca gaggaagaga gttcactcga gcacggagtc aataaacagc 661 tgggtgatat gtagagtgtt cgaatcaagc tgcggttcgc aagtgagctt ccatgacgag 721 ggcacagagc tttcgtgctt ggatgaggtg ttcttgtctc tagacgacta cgacgaagtg 781 agcttgccaa ataataaggg tgggcgcgcc gacccagctt tcttgtacaa agttggcatt 841 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa 901 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct 961 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa 1021 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt 1081 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat 1141 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc 1201 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc 1261 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg 1321 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc 1381 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat 1441 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac 1501 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat 1561 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg 1621 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat 1681 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc 1741 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca 1801 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag 1861 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg 1921 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg 1981 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 2041 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 2101 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 2161 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 2221 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 2281 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 2341 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 2401 accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga 2461 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 2521 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2581 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2641 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2701 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2761 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 2821 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 2881 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag 2941 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg 3001 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat 3061 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac 3121 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact 3181 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt 3241 cttaagctcg ggccc //