LOCUS Exported 1069 bp ds-DNA linear SYN 13-JUN-2021 DEFINITION Expresses 3Xflag::tagRFP::myc in bacteria. ACCESSION . VERSION . KEYWORDS AP575-1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1069) AUTHORS Paix A, Folkmann A, Rasoloson D, Seydoux G TITLE High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes. JOURNAL Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. Epub 2015 Jul 17. PUBMED 26187122 REFERENCE 2 (bases 1 to 1069) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1069 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(52..81) /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS complement(82..789) /codon_start=1 /product="monomeric derivative of red fluorescent protein from Entacmaea quadricolor (Merzlyak et al., 2007)" /label=TagRFP /note="mammalian codon-optimized" /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDM ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVA RYCDLPSKLGHKLN" CDS complement(790..855) /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" primer_bind complement(882..899) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(882..898) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(891..913) /label=M13/pUC Forward /note="In lacZ gene" ORIGIN 1 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat ccagatcctc 61 ttctgatatc agtttttgtt cattaagttt gtgccccagt ttgctaggga ggtcgcagta 121 tctggccaca gccacctcgt gctgctcgac gtaggtctct ttgtcggcct ctttgattct 181 ttccagtctg tggtccacat agtagacgcc gggcatcttg aggttcttag cgggtttctt 241 ggatctgtat gtggtcttga aattgcagat caggtggccc ccgcccacga gcttcagggc 301 catgtcgctt ctgccttcca ggccgccgtc agcggggtac agcatctcgg tattggcctc 361 ccagccgagt gttttcttct gcatcacagg gccgttggat gggaagttca cccctctgat 421 cttgacgttg tagatgagac agccgtcctg gaggctggtg tcctgggtag cggtcagcac 481 gcccccgtct tcgtatgtgg tgactctctc ccatgtgaag ccctcaggaa aggactgctt 541 aaagaagtcg gggatgccct gggtgtggtt gatgaaggtt ctgctgccgt acatgaagct 601 ggtagccagg atgtcaaagg cgaaggggag agggccgccc tcgaccacct tgattctcat 661 ggtctgggtg ccctcgtagg gcttgccttc gccctcggat gtgcatttga agtggtggtt 721 gttcacggtg ccctccatgt acagcttcat gtgcatgttc tccttaatca gctcttcgcc 781 cttagacacc ttgtcatcgt catccttgta atcgatatca tgatctttat aatcaccgtc 841 atggtctttg tagtcgatcc ccgggtaccg agctcgaatt cactggccgt cgttttacaa 901 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct 961 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc 1021 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatc //