LOCUS Exported 3465 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1169-HQ858713 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3465) TITLE Maize TF ORFeome collection REFERENCE 2 (bases 1 to 3465) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3465 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1022..1121) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1138..1157) /label=T7 /note="T7 promoter, forward primer" promoter complement(1139..1157) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1162..1178) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1162..1178) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1280..1299) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1291..2100 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1358..1377) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2247..2835 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2736..2755 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2989..3006 /label=L4440 /note="L4440 vector, forward primer" terminator 3165..3192 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3284..3370 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3419..3441 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3433..3450 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3434..3450 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc 121 atgagcggcg ccggtccgga tctgcagctg ccaccggggt tccggttcca cccgacggac 181 gaggagctgg tgatgcacta cctctgccgc cgctgcgccg gcctgcccat cgccgtcccc 241 atcatcgccg agatcgacct ctacaagttc gacccatggc agctcccaag gatggcgctg 301 tacggcgaga aggagtggta cttcttctcc ccgcgggacc gcaagtaccc gaacgggtcc 361 aggcccaacc gcgccgccgg ggctgggtac tggaaggcca ccggcgctga caagcccgtg 421 ggcacgccca agccgctggc catcaagaag gcgctcgtct tctacgccgg caaggcgccc 481 aagggcgaga agaccaactg gatcatgcac gagtaccgcc tcgccgacgt cgaccgctcg 541 gcgcgcaaga agaacagcct caggttggat gactgggtcc tgtgccgcat ctacaacaag 601 aagggcggcg ggctggagaa ggcggcggcg ccggcggccg gcggcgacca caagcctgtg 661 ttcgccacgg cggcggtgag ctccccgccg gagcagaagc cgttcgtggc ggcggcgggc 721 gggctgcccc cggcgttccc ggagctggcg gcgtactacg accggccgtc ggactcgatg 781 ccgcggctgc acgcggacta ctccagctgc tcggagcagg tgctgtcccc ggagcagctg 841 gcgtgcgacc gggaggtgca gagccagccc aagatcagcg agtgggagcg gaccttcgcc 901 tccgaccccg tgaaccccgc gggctccatg ctcgaccccg tcgtcggcca cgccggcggc 961 gacccgctgc tgcaggacat cctcatgtac tggggcaagc cgttcaaggg tgggcgcgcc 1021 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc 1081 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc 1141 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa 1201 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct 1261 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg 1321 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat 1381 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg 1441 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta 1501 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat 1561 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa 1621 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat 1681 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg 1741 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc 1801 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca 1861 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata 1921 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta 1981 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt 2041 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa 2101 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc 2161 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac 2221 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 2281 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 2341 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 2401 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 2461 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 2521 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 2581 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat 2641 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 2701 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 2761 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 2821 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 2881 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 2941 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 3001 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 3061 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 3121 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca 3181 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc 3241 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga 3301 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc 3361 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc 3421 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc //