LOCUS Exported 3213 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1396-HQ858728 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3213) TITLE Maize TF ORFeome collection REFERENCE 2 (bases 1 to 3213) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3213 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" RBS 112..132 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(770..869) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(886..905) /label=T7 /note="T7 promoter, forward primer" promoter complement(887..905) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(910..926) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(910..926) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1028..1047) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1039..1848 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1106..1125) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 1995..2583 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2484..2503 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2737..2754 /label=L4440 /note="L4440 vector, forward primer" terminator 2913..2940 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3032..3118 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3167..3189 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3181..3198 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3182..3198 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac 121 tttaagaagg agcccttcac catgtctttc accggcacgc aggacaagtg caaagcctgc 181 gacaagacgg tccacatcat cgacctcctc accgccgacg gcgtctcgta ccacaagaca 241 tgcttcaagt gcagccactg caagggcgtc ctctcgatta gcagctactc ttccatggac 301 ggcgtcctgt actgcaagac gcactttgaa cagctcttca aggagacagg gaccttctca 361 aagaactttc aaggtggagc atcttcaaac aagaacgacc aggcaaaggc tccaagcaag 421 ctatcatctg cattctctgg aactcaagat aaatgcgcgg cctgccagaa aacagtgtat 481 ccattggaga agatgactct ggaaggcgaa tcctaccaca agagctgctt caaatgctcg 541 cacgggggct gcatcctcac cacgtcctcc tacgccgcgc tcaacggggt cctctactgc 601 aagatccatt tctcgcagct gttcaaggag aagggcagct acaaccacct catcgagacg 661 gcgcagacca agaagaacga ggccgcggag gccggaccgg agccaccggc ggacgcaggc 721 gcggctgagc cggaagcacc cgcgcaatcg gcgaagggtg ggcgcgccga cccagctttc 781 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc 841 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg 901 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt 961 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac 1021 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat 1081 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca 1141 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat 1201 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg 1261 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta 1321 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca 1381 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt 1441 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg 1501 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa 1561 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat 1621 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat 1681 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc 1741 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct 1801 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt 1861 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga 1921 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag 1981 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 2041 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 2101 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 2161 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 2221 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 2281 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 2341 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc 2401 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 2461 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 2521 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 2581 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 2641 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 2701 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 2761 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 2821 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt 2881 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct 2941 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt 3001 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg 3061 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat 3121 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt 3181 tgtaaaacga cggccagtct taagctcggg ccc //