LOCUS Exported 3351 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1434-HQ858736 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3351) TITLE Maize TF ORFeome collection REFERENCE 2 (bases 1 to 3351) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3351 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" RBS 112..132 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(908..1007) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1024..1043) /label=T7 /note="T7 promoter, forward primer" promoter complement(1025..1043) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1048..1064) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1048..1064) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1166..1185) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1177..1986 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1244..1263) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2133..2721 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2622..2641 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2875..2892 /label=L4440 /note="L4440 vector, forward primer" terminator 3051..3078 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3170..3256 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3305..3327 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3319..3336 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3320..3336 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac 121 tttaagaagg agcccttcac catggatatg gcccccgcgg ccgtctcctc caacccgcgc 181 tcggtcgagg aaatcttcaa agacttctcc ggccggcgcg cggggctcgt ccgcgccctc 241 acctccgatg tggacgattt ctgcagcttc tgcgacccag ataaggagaa cttgtgtctc 301 tacggccttc ccaacggtag ctgggaggtc tcgccgccgg cggacgaggt tcctccggag 361 ttgcccgagc cggcgctcgg catcaacttt gcgcgcgatg gcatgcagcg ccgcgactgg 421 ctctcactcg tcgcggtcca ctctgactcg tggctcatct cagtcgcctt cttctacggc 481 gcccgcctca acgccaacga ccggaagcgc ttattcagca tgatcagtga tcttccttct 541 gtctttgaag catttgcgga caggaaacac gtcagggata ggtctggcgt tgatagcagc 601 ggcaagtcca ggcactcatc aaagagagga aacgatggcc atgcgaagaa ctccagggca 661 gcagctcctg ctgccaaaga gtacgacgac gacgatgacg aggaggacga ggaacacacg 721 gaaacctttt gtggaagctg cggcggcctc tacaacgcga acgagttctg gatcggctgc 781 gacatctgcg agcggtggtt ccacgggaag tgcgtgcgga tcacccctgc caaagcggac 841 cacataaagc actacaagtg cccggactgc agctcgaaga aaataaggca gaagggtggg 901 cgcgccgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt 961 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat 1021 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg 1081 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa 1141 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg 1201 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc 1261 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca 1321 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc 1381 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact 1441 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta 1501 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg 1561 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct 1621 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt 1681 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg 1741 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa 1801 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc 1861 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa 1921 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt 1981 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg 2041 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg 2101 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 2161 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 2221 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc 2281 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 2341 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 2401 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 2461 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 2521 gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 2581 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 2641 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 2701 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 2761 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 2821 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 2881 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg 2941 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 3001 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat 3061 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc 3121 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac 3181 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag 3241 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg 3301 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc c //