LOCUS       Exported                 761 bp ds-DNA     linear   SYN 25-MAY-2021
DEFINITION  Retroviral expression of Y705F-STAT3. Please note that this plasmid 
            contains Y705F-STAT3 tagged with FLAG, not GFP..
ACCESSION   .
VERSION     .
KEYWORDS    pLEGFP-Y705F-STAT3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Dasgupta M, Unal H, Willard B, Yang J, Karnik SS, Stark GR
  TITLE     Critical role for lysine 685 in gene expression mediated by 
            transcription factor unphosphorylated STAT3.
  JOURNAL   J Biol Chem. 2014 Oct 31;289(44):30763-71. doi: 
            10.1074/jbc.M114.603894. Epub 2014 Sep 12.
  PUBMED    25217633
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 25, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      10..19
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             complement(74..97)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccttgctcac catggtggcg accggtggat cccgggcccg cggtaccgtc gactgcagaa
       61 ttcgaagctt tcacttgtca tcgtcgtcct tgtagtccat gggggaggta gcgcactccg
      121 aggtcaactc catgtcaaag gtgagggact caaactgccc tcctgctgag ggttcagcac
      181 cttcaccatt atttccaaac tgcatcaatg aatctaaagt gcggggggac atcggcaggt
      241 caatggtatt gctgcaggtc gttggtgtca cacagataaa cttggtcttc aggaatgggg
      301 cagcgctacc tgggtcagct tcaggatgct cctggctctc tggccgacaa tactttccga
      361 atgcctcctc cttgggaatg tcaggataga gatagaccag tggagacacc aggatattgg
      421 tagcatccat gatcttatag cccatgatga tttcagcaaa tgacatgttg ttcagctgct
      481 gctttgtgta tggttccacg gactggatct gggtcttacc gctgatgtcc ttctccaccc
      541 aagtgaaagt gacgcctcct tctttgctgc tttcactgaa tcttagcagg aaggtgcctg
      601 gaggcttagt gctcaagatg gcccgctccc gctccttact gataaagccc atgatgtacc
      661 cttcgttcca aagggccagg atgtactttt tcacaaggtc aatgatattg tccagccaga
      721 cccagaagga gaagcccttg ccagccatgt tttctttgca a
//
