LOCUS Exported 3666 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1460-HQ858751 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3666) TITLE Maize TF ORFeome collection REFERENCE 2 (bases 1 to 3666) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3666 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" RBS 112..132 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(1223..1322) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1339..1358) /label=T7 /note="T7 promoter, forward primer" promoter complement(1340..1358) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1363..1379) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1363..1379) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1481..1500) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1492..2301 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1559..1578) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2448..3036 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2937..2956 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3190..3207 /label=L4440 /note="L4440 vector, forward primer" terminator 3366..3393 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3485..3571 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3620..3642 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3634..3651 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3635..3651 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac 121 tttaagaagg agcccttcac catgacgagc ggcgccgggg gagccgcggc cgggatcggg 181 ggcaccaggg tgcccacgtg gagggagcgc gagaacaacc gccgcaggga gcgccggcgc 241 cgcgcgatcg ccgccaagat cttcgccggc ctcagggcct acggcaacta caacctgccc 301 aagcactgcg acaacaacga ggtgctcaag gcgctctgca acgaggccgg ctggaccgtc 361 gagcccgacg gcaccaccta ccgcaaggga tgtaaacctc tggcaacaga gcgtccagat 421 ccaattggga ggtctgcatc accaagcccc tgctcttcat atcaaccaag tccgcgagcc 481 tcatacaacc caagcgcggc atcctcctcg ttcccaagct ctgggtcctc ctcccacatt 541 actcttggcg ggagcaactt catgggaggc gtcgagggca gctcccttat cccgtggcta 601 aagaacctct cgtcgagctc ctcgttcgcc tcctcctcca agttcccgca gcttcaccac 661 ctctacttca acggcggttc catcagcgcg ccggtgacgc ctccatccag ctctccaacc 721 cgcacgcctc gcatcaagac tgactgggag aacccgagtg ttcagccacc gtgggctggg 781 gcgaactacg cgtctcttcc caactcccag ccgccgagcc ctgggcacca ggttgctccg 841 gacccggcgt ggctagccgg gttccagatt tcgtctgctg gcccttcgtc tccaacttac 901 agccttgtgg ctccgaatcc gtttggtatc ttcaaggaga ccatcgtcag cacctcaaga 961 atgtgcaccc ctgggcagag tggaacgtgc tctcctgtaa tgggcggtgc gccgatccat 1021 cacgatgtcc agatggctga tggtgcccca gatgacttcg ccttcgggag cagcagcaac 1081 ggcaacaacg agtcgcctgg tctcgtgaag gcatgggagg gggaacggat acacgaggag 1141 tgcgcctcgg acgagcatga gctggagctc acccttggga gctcaaagac tcgtgcagat 1201 ccttccaagg gtgggcgcgc cgacccagct ttcttgtaca aagttggcat tataagaaag 1261 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt 1321 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg 1381 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca 1441 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat 1501 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg 1561 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg 1621 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt 1681 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag 1741 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca 1801 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca 1861 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc 1921 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat 1981 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt 2041 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt 2101 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga 2161 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa 2221 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg 2281 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt 2341 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg 2401 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 2461 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 2521 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 2581 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 2641 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 2701 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 2761 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 2821 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac 2881 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 2941 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 3001 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 3061 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 3121 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 3181 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 3241 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 3301 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa 3361 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg 3421 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg 3481 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc 3541 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta 3601 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc 3661 gggccc //