LOCUS Exported 3363 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1461-HQ858752 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3363) TITLE Maize TF ORFeome collection REFERENCE 2 (bases 1 to 3363) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3363 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" RBS 112..132 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(920..1019) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1036..1055) /label=T7 /note="T7 promoter, forward primer" promoter complement(1037..1055) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1060..1076) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1060..1076) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1178..1197) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1189..1998 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1256..1275) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2145..2733 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2634..2653 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2887..2904 /label=L4440 /note="L4440 vector, forward primer" terminator 3063..3090 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3182..3268 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3317..3339 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3331..3348 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3332..3348 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac 121 tttaagaagg agcccttcac catgaggaag aagctcggca cccggttccc cgcggctcga 181 atcaaaaaga taatgcaagc agatgaggat gttggaaaga ttgcactagc agttcctgtt 241 ttagtttcga ggtctcttga attgttttta caagatttaa ttgaccgtac ttatgaaatt 301 actcttcaaa gtggagcaaa gacactgaat tccttccacc tgaagcaatg tgtgaagagg 361 tacagttctt ttgatttcct aactgaagtt gtcagcaagg taccagatct tggtggcgct 421 gattcctgtg gagatgaaag aggattacct agaagaagaa agtcaaatgg cagtgaccca 481 gagaatgatg aatcaagatc tagtaaaatg gccataagaa atgcaaacat cagccccaga 541 ggacgtggga ggggtcgagg cagaggacga ggtcggccac caaccaagag aaaggaggtt 601 ggttatgtac aatttgaaga tgagagcagc atgtttgctg aacaaggtga gcccttgcca 661 ggagaggaaa cagttcaaga gatcaatggc aacgagacca tgcctcaaag cacgcaacct 721 ccagtagagg ctccgccaac agcccttgca caagctacaa caagttntaa ggcggaagaa 781 gcgaacagtg atcatcagtc agattggcct atgccagatg ccattggaag catcggtgtc 841 gtgccatctg gttttggaca tctgacagtg caggttgaag atgaggacta cgacaatgag 901 gataagggtg ggcgcgccga cccagctttc ttgtacaaag ttggcattat aagaaagcat 961 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc 1021 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag 1081 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca 1141 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt 1201 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat 1261 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag 1321 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca 1381 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat 1441 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca 1501 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg 1561 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta 1621 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt 1681 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg 1741 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt 1801 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac 1861 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg 1921 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg 1981 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg 2041 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg 2101 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 2161 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 2221 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 2281 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 2341 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 2401 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 2461 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 2521 tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 2581 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 2641 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 2701 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 2761 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 2821 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 2881 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc 2941 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 3001 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg 3061 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg 3121 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg 3181 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt 3241 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg 3301 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg 3361 ccc //