LOCUS Exported 3750 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1490-HQ858754 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3750) TITLE Maize TF ORFeome collection REFERENCE 2 (bases 1 to 3750) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3750 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" RBS 112..132 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(1307..1406) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1423..1442) /label=T7 /note="T7 promoter, forward primer" promoter complement(1424..1442) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1447..1463) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1447..1463) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1565..1584) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1576..2385 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1643..1662) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2532..3120 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3021..3040 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3274..3291 /label=L4440 /note="L4440 vector, forward primer" terminator 3450..3477 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3569..3655 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3704..3726 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3718..3735 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3719..3735 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac 121 tttaagaagg agcccttcac catggggaag gcggaggcga ggtcaagggg gcgcggcggc 181 ggcggcggcg gcggaggcgc cagcggcaat ccggggaaga tcttcgtcgg agggctcccg 241 cgggacacca cggatgctac atttgtgagg cattttggac agtatggaga aatagttgat 301 tcggtgataa tgaaggatag gcacacatct cagccacggg gttttggctt cattacatac 361 tctgaccctg ctgttgttga taaagtgata gaggacaatc atgttatcaa tggaaagcaa 421 gttgaaatta agagaaccat accaaaaggt tcggtgcaat ctagctctaa ggatttcaag 481 actaaaaaga tatttgttgg tgggcttccg tcaacactaa cagaagacga attcaagagt 541 ttctttgcaa gatatgggac tgtggtggac caccagatta tgtttgatca tgaaaccaaa 601 cgttcccgag gctttggatt tatagttttt gcttctgagc aagttgtaga tgatttgtta 661 gcaaatggaa atatggttga ccttgctggt tcaaaggtgg agatcaagaa agcagaacca 721 aagaaatcct ccaatgcacc gccaccacca gtgcatgtta gaaatgctag gtcagcatat 781 gatagcggct ctagagacca cccttcagct gacaactatg gtggaatggc cagtgcttat 841 ggaaactaca ggggtggtgg atttggtcct tataggagcg atacaggatt tggtgctagg 901 ctcagcagct atggtggggt aggtgatttt ggtggtgcat atggtcgtta ctatgcagga 961 ttaggagggg gatatggagc cgcgtcttcg tttggttatc ctggtcgctt tgggttatat 1021 ggaggaggtt ttggtggatc ttatgctggt gctgacttaa gtggctatag acgcggtgga 1081 ggtgatgaga gcttcggtgc ccctggtggt aatcctgggt ttggtggtga tgctgaagag 1141 agctttggtg gccctagtag ttctggcttt agtggcaccg cctatggtgg ggcatatgat 1201 cctacgctag gtggttatgg atctgctagc acacctgaca tgaatagggg aagcttcact 1261 ggaggatatg gacgatatca cccatatgga aagggtgggc gcgccgaccc agctttcttg 1321 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact 1381 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat 1441 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac 1501 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt 1561 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc 1621 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt 1681 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc 1741 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa 1801 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc 1861 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt 1921 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt 1981 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat 2041 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa 2101 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt 2161 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt 2221 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt 2281 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat 2341 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat 2401 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca 2461 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc 2521 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 2581 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 2641 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 2701 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 2761 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 2821 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 2881 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg 2941 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 3001 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 3061 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 3121 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 3181 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 3241 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 3301 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 3361 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc 3421 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct 3481 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct 3541 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca 3601 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc 3661 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt 3721 aaaacgacgg ccagtcttaa gctcgggccc //