LOCUS Exported 3294 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1757-HQ858771 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3294) TITLE Maize TF ORFeome collection REFERENCE 2 (bases 1 to 3294) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3294 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" RBS 112..132 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(851..950) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(967..986) /label=T7 /note="T7 promoter, forward primer" promoter complement(968..986) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(991..1007) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(991..1007) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1109..1128) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1120..1929 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1187..1206) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2076..2664 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2565..2584 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2818..2835 /label=L4440 /note="L4440 vector, forward primer" terminator 2994..3021 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3113..3199 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3248..3270 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3262..3279 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3263..3279 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac 121 tttaagaagg agcccttcac catgcaggca tatatggagg gaggccagtt gttgggggct 181 tgctgccttc ctggcttcct tgtgccggat cactacgccg ccttccctct tcctctcccg 241 ctacaacttc ctagccagcc aaacgataat aggcttttcc agatgccgtt tgaccaggaa 301 gaagccgaga accatggcgg gatgctctac tccgaccaat gtggactata cccgctgccg 361 gccttcggca gctgctctgc tgccgccggc gcaacagcga agcctactgc tggtttcatg 421 cccagtacta ttggcgctga gacgaaggtc tgcacctcgc tgactactaa aggttgcaac 481 gagagtaata gcacatggtg gaaggggtca gcagctacga tagcggagag agggaagatg 541 aaggtgagga ggaagatgag ggaaccgagg ttttgcttcc agaccagaag cgatgtggat 601 gtactggatg atggctacaa gtggaggaag tacgggcaga aggttgtcaa gaacagcctc 661 catccaagga gctatttccg gtgcacccac agcaactgcc gcgtgaagaa acgggtggag 721 cggctgtcga cggactgccg catggtgatg accacgtacg agggccgcca cacgcactct 781 ccctgcagcg acgacgcttc ctccggcgac cacaccgatt gcttcagctc ctttaagggt 841 gggcgcgccg acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca 901 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct 961 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc 1021 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata 1081 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa 1141 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct 1201 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg 1261 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg 1321 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt 1381 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta 1441 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc 1501 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc 1561 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag 1621 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca 1681 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg 1741 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt 1801 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa 1861 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag 1921 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga 1981 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga 2041 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 2101 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 2161 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 2221 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 2281 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 2341 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 2401 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 2461 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 2521 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 2581 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 2641 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 2701 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 2761 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 2821 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt 2881 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 2941 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc 3001 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg 3061 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc 3121 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact 3181 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg 3241 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc //