LOCUS Exported 3360 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1760-HQ858774 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3360) TITLE Maize TF ORFeome collection REFERENCE 2 (bases 1 to 3360) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3360 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" RBS 112..132 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(917..1016) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1033..1052) /label=T7 /note="T7 promoter, forward primer" promoter complement(1034..1052) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1057..1073) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1057..1073) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1175..1194) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1186..1995 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1253..1272) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2142..2730 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2631..2650 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2884..2901 /label=L4440 /note="L4440 vector, forward primer" terminator 3060..3087 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3179..3265 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3314..3336 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3328..3345 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3329..3345 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac 121 tttaagaagg agcccttcac catggcaatc gacgacgaat cgccgatcag agtcaacagc 181 agaggcggcg gcgccatggg aggaggggag tgcgacgggg cggagaacca gcggtggccg 241 ccgtggctca agccgctgct ggggacgagc ttcttcggcc aatgcaagct gcacgcggac 301 gcgcacaaga gcgagtgcaa catgtactgc ctcgcctgca tgaacggcgc gctctgctcc 361 cagtgcctcg cctaccaccg cgaccaccac gccatccaga tacggaggtc gtcctaccac 421 gacgtgatcc gggtgtcgga gatccagaag gtgctggaca tctccggcgt gcagacgtac 481 atcatcaaca gcgcgcgcgt ggtgttcctg aacgagcgcc cgcagcagag gccaggcaag 541 ggcgtcacca acacctgcga ggtctgccag cgcagcctcc tcgacacctt ccgcttctgc 601 tccctcgggt gcaagatcgt ggggacctcc ggtgacttgc gcatccggaa gaagcaggcc 661 gttgtcaaga agcatcagaa gaagaagaag cagcaggcgc agcagcacag gggcgccgcg 721 ttggactcgg aggacgactc ttcgtccacc agcaccagcc gcggcagcga caggagcagc 781 gtggtgcaga gcttcacccc gtcgaccccg ccggcgacgg ccaacagctt ccgaaccggg 841 aagcggcgca agggcgtgcc gcaccggtcg ccgttcggca gcctcatggt ggaggagttc 901 aagggtgggc gcgccgaccc agctttcttg tacaaagttg gcattataag aaagcattgc 961 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat 1021 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc 1081 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga 1141 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa 1201 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa 1261 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc 1321 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat 1381 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt 1441 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc 1501 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc 1561 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt 1621 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat 1681 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca 1741 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac 1801 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag 1861 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt 1921 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc 1981 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg 2041 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc 2101 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 2161 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2221 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2281 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2341 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 2401 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 2461 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 2521 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 2581 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 2641 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 2701 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 2761 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 2821 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 2881 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc 2941 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 3001 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa 3061 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc 3121 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat 3181 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt 3241 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta 3301 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc //