LOCUS       Exported                9374 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses the extracellular region of the PlexinA3 protein, 
            C-terminally fused to the Fc region of human IgG1 + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    Plxna3-Fc-His
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9374)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9374
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        105..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             5954..5971
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5997..6118
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6034..6053)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6088..6107
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6156..6173)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6327..6915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6407..6426)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7086..7946)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7709..7728
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7947..8051)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8119..8137
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8175..8197)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8199..8219)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8204..8534
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8385..8520
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8447..8466
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8671..8690
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8757..9212)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8894..8915)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9106..9125
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9338..9360
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9352..9369
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9353..9369
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgcccactgt
     1621 ctgccttctc ccattgctat tcttcaccat aggagggtgc ctgggtagca gcaggccatt
     1681 tcgtaccttt gtggtaacag ataccactct gactcacctg gctgtgcacc gagtgactgg
     1741 ggaggtgttt gtaggtgccg tgaatcgagt tttcaagctg gctccaaacc taactgagct
     1801 acgggcccat gtcacagggc ccattgagga caatgctcgc tgctacccac ctcctagcat
     1861 gcgtgtgtgc tcccaccgcc tggtgcctgt ggacaatgtg aacaagctgc tcctcataga
     1921 ctatgcagcc cgtcgtttgg tagcttgtgg cagcatctgg cagggcatct gccagttcct
     1981 gcgtctggat gacctcttca agttgggtga gccccaccat cgcaaggagc actatctgtc
     2041 aggggcccag gagcctgatt ccatggctgg cgtcattgtt gaacaggtcc aggggcctag
     2101 caagttgttt gtgggcaccg ctgttgatgg caagtctgag tactttccca ccttgagttc
     2161 ccgtaagctc atcgacgatg aggacagtgg agatatgttc agtctggtgt accaggatga
     2221 gtttgtctct tctcagatca agatcccttc agacacattg tccttgtacc ctgcctttga
     2281 catctactac atatatggct ttgtcagtgc ctccttcgtg tacttcttaa cgttgcagct
     2341 ggacacccag cagacgctgt tggatacagc aggcgagaaa ttcttcacat ccaagatcgt
     2401 gcgcatgtgt gcaggggact cagagttcta ctcttatgta gagttcccta ttggctgctc
     2461 ctggcgtggt gtggagtacc gcttggtgca gagtgcccat ctggccaagc caggcctgct
     2521 attggcccag gctctgggtg tgccagccga cgaggatgtc ctcttcacca tcttctctca
     2581 gggccagaag aacagagcca acccacctcg gcagaccatc ctttgccttt tcaccctcag
     2641 cagcattaat gcccacatcc ggcgccgaat ccaatcgtgc taccgtggag agggcacgct
     2701 ggccctaccc tggctactga ataaggagct gccctgcatc aacacccctc tgcagatcaa
     2761 tggaaacttc tgtgggttag tgttgaatca accattgggt ggcctacacg tgattgaggg
     2821 gctacccttg ctggctgaca gcactgatgg catggccagt gtggctgcct acacctacca
     2881 ccagcattct gtggttttca tcggtacacg cagcggtaat ttgaagaagg tgcgggtcga
     2941 tggctctcag gatgcccagc tatatgaaac ggtctctgtg gtgcagggca gccccatact
     3001 tcgagacctg ctcttcagcc ctgaccaccg acacatctac ctcctgagtg agaagcaggt
     3061 gagccaactc ccggtggaga cctgtgagca gtatctgagc tgtgctgcat gcctgggctc
     3121 aggggaccca cactgtggtt ggtgtgtgct acagcacagg tgctgccgtg aaggggcctg
     3181 tccaggtgcc tctgccccac atggctttgc agaagagctg agcaaatgta tacaggtgcg
     3241 ggtccgtccc aataatgtgt cagtgacatc ctctggggtg cagctgaccg tagccatgcg
     3301 caacgtgcca gacctcagtg tgggtgtgag ctgttccttt gaggaggtga ctgaaagtga
     3361 ggctattctg ctgccctctg gagagttacg atgtccttca ccatcccttc aggagctcca
     3421 gacacttacc agagggcatg gggccactca tactgtgcgg cttcagctgc tctccatgga
     3481 gactggtgtg aggtttgctg gggttgactt tgtcttctac aactgcagtg ccctccagtc
     3541 gtgtatgtcc tgtgttggca gcccttaccc ctgccactgg tgtaagtacc gtcatgtgtg
     3601 taccagccac ccacacgagt gctctttcca ggagggcagg gtccacagcc ctgagggctg
     3661 ccctgagatc ctgcctcaag gggacctctt gattcctgtg ggtgtcatgc agcctctaac
     3721 tctgcgggct aagaacctgc cacagcctca gtccggacag aagaattatg aatgcgtggt
     3781 ccgtgtacag ggacggcaac atcgggtacc tgcagtgcgc tttaacagca gcagtgtgca
     3841 gtgccagaat gcctcgtact tctatgaagg tgatgagttt ggtgacaccg aactggactt
     3901 ctctgtggtt tgggatggag atttccccat tgataagcct cctagcttcc gagcccttct
     3961 ttacaagtgc tgggctcagc ggcctagctg tggcctctgc ctcaaggctg atccccgatt
     4021 caactgtggc tggtgcatct cagagcacag gtgccagctg agggctcact gcccagctcc
     4081 caaaagtaac tggatgcacc caagccagaa gggtgcccga tgcagccatc cccgaatcac
     4141 ccagattcat ccacttacag gacccaagga gggtggcacc cgggtcacca ttgtgggtga
     4201 gaacctgggc ctcacttccc gtgaggttgg ccttcgagta gctggtgtac gttgcaactc
     4261 cattcccacc gaatatgtca gtgctgaaag gatcgtatgt gagatggagg aatcgctggt
     4321 gcccagccca ccacctgggc ctgctgagct ctgtgtaggg gattgttctg ctgacttccg
     4381 cacacagtcc cagcaactct acagctttgt gacccctaca tttgaccgtg tgagtccctc
     4441 tcggggccca gcttctggag gcactcggct taccatctct ggaatttctc tggatgccgg
     4501 cagcagggtc acagtgatta taagagatgg cgagtgccag tttgtgagga gagatgccga
     4561 ggcaatcgtg tgtatctcac ctgtctccac cctgggcccc agtcagagcc ctatcaccct
     4621 tgccatcgat catgccaaca tctccaacac tggagtcatc tatacctaca cccaggaccc
     4681 tacggtcaca caccttgagc ccacctggag catcatcaat ggaagcacct ccatcactgt
     4741 gagtggaacc catctgctga cagttcaaga accccgtgta cgagccaagt accgtggtat
     4801 tgagaccact aacacatgcc aggtgatcaa cgacactgca atgctatgta aggcccctgg
     4861 catcttcctt gggcaccctc agcctcgggc ccaaggcgag caccctgacg agtttggctt
     4921 cttgctggac cacgtgcagg cagcccgctc cctcaaccgt tcttccttca cctactaccc
     4981 tgatcccagc tttgaaccac ttgggccctc tggtgtgcta gatgtcaaac ctggttcaca
     5041 tgttgtattg aagggcaaga acctgatccc tgctgcagct ggcagctccc gcctcaacta
     5101 cacagtgttg attggaggac agccatgtgc actaactgtc tcagatactc aacttctgtg
     5161 tgattcccca agccagacag gccggcagcc tgttatggtg ctggtgggtg gcctggagtt
     5221 ctggttgggc acgctgcaca tcactgctga tcgggcactg actagtgaca aaactcacac
     5281 atgcccaccg tgcccagcac ctgaactcct ggggggaccg tcagtcttcc tcttcccccc
     5341 aaaacccaag gacaccctca tgatctcccg gacccctgag gtcacatgcg tggtggtgga
     5401 cgtgagccac gaagaccctg aggtcaagtt caactggtac gtggacggcg tggaggtgca
     5461 taatgccaag acaaagccgc gggaggagca gtacaacagc acgtaccgtg tggtcagcgt
     5521 cctcaccgtc ctgcaccagg actggctgaa tggcaaggag tacaagtgca aggtctccaa
     5581 caaagccctc ccagccccca tcgagaaaac catctccaaa gccaaagggc agccccgaga
     5641 accacaggtg tataccctgc ccccatcccg ggatgagctg accaagaacc aggtcagcct
     5701 gacctgcctg gtcaaaggct tctatcccag cgacatcgcc gtggagtggg agagcaatgg
     5761 gcagccggag aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt
     5821 cctctacagc aagctcaccg tggacaagag caggtggcag caggggaacg tcttctcatg
     5881 ctccgtgatg catgaggctc tgcacaacca ctacacgcag aagagcctct ccctgtctcc
     5941 gggtaaaggc ggccatcatc accatcacca ttaatctaga gatcttatta aagcagaact
     6001 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6061 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6121 atgtctggtc gactctagct agactcttcc gcttcctcgc tcactgactc gctgcgctcg
     6181 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6241 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6301 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6361 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6421 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6481 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6541 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6601 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6661 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6721 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6781 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6841 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6901 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6961 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7021 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7081 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7141 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7201 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7261 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7321 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7381 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7441 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7501 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7561 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7621 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7681 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7741 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7801 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7861 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7921 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7981 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8041 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     8101 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     8161 gatgacggtg aaaacctctg acacatgcag ctcccggagg aatgtgtgtc agttagggtg
     8221 tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt
     8281 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     8341 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     8401 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     8461 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     8521 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcgtcccg gagacggtca
     8581 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     8641 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     8701 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     8761 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     8821 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     8881 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     8941 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     9001 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     9061 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     9121 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     9181 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
     9241 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     9301 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     9361 acggccagtg aatt
//
