LOCUS Exported 3507 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1771-HQ858785 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3507) TITLE Maize TF ORFeome collection REFERENCE 2 (bases 1 to 3507) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3507 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" RBS 112..132 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(1064..1163) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1180..1199) /label=T7 /note="T7 promoter, forward primer" promoter complement(1181..1199) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1204..1220) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1204..1220) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1322..1341) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1333..2142 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1400..1419) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2289..2877 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2778..2797 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3031..3048 /label=L4440 /note="L4440 vector, forward primer" terminator 3207..3234 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3326..3412 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3461..3483 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3475..3492 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3476..3492 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac 121 tttaagaagg agcccttcac catgacggag ctgtggatgg aggtgctgcc gccgccctac 181 ttcgcggggc aggcggcggg cgggtggttc ctgccggacc ggcgggccgg gcccggggcg 241 tggacgctgg aggagaacaa gatgttcgag cgcgcgctgg cgagggtcga ctgggacgcg 301 ccggaccggt gggagcgggt ggccgcgttg ctgcccggca ggacggcgtc cgacgtcgcg 361 gcccactacg acgacctgga gtgcgatgtc ggctgcatcg aggcaggctt cgtgcccttc 421 ccctgctacg gcagcggcgg cggcgcgtcg cagtccgccg ggttcacgtt cgactgggac 481 gccggcgggc tgggctttaa gcgctcctgc tacgtggtcg gcggcggcaa gcgcgagcgc 541 gggccggacc aggagcgcaa gaagggcgtc ccctggacgg aggaagagca caagcagttc 601 ttgatgggcc tgaagaagta cgggcgaggg gactggagga acatctcgcg caacttcgtg 661 acgagccgga cgccgacgca ggtggccagc cacgcgcaga agtacttcat ccgcctcaac 721 tccggcggca aggacaagcg ccgctccagc atccacgaca tcaccaccgt caacctcccc 781 gacgacgacg cgggcggcac cgcctccgcc tcgccgccct ccgtgctcac ctccgcctcc 841 gcgccctcgt ccaccggcgg cggcggcccg gtcgtctccg agcagttcgg cgtgctcgtc 901 gacagcaagc ccccggcgcc gcaccaccac ttcgtgccgc acccgtacgg caacgtgaag 961 ctcgagcccg tcagttccca ccacggcggg ttcctggacg actctgtcct gatgcagatg 1021 cactgcgggc agctgcagcc tctcggaaag ggtgggcgcg ccgacccagc tttcttgtac 1081 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc 1141 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac 1201 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt 1261 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat 1321 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac 1381 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg 1441 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa 1501 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt 1561 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc 1621 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa 1681 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat 1741 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac 1801 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc 1861 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat 1921 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga 1981 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag 2041 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg 2101 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg 2161 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa 2221 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa 2281 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 2341 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 2401 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 2461 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 2521 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 2581 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 2641 cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt 2701 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 2761 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 2821 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 2881 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 2941 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 3001 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 3061 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 3121 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct 3181 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag 3241 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca 3301 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac 3361 aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg 3421 cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa 3481 acgacggcca gtcttaagct cgggccc //