LOCUS Exported 3591 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1803-HQ858789 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3591) TITLE Maize TF ORFeome collection REFERENCE 2 (bases 1 to 3591) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3591 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" RBS 112..132 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(1148..1247) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1264..1283) /label=T7 /note="T7 promoter, forward primer" promoter complement(1265..1283) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1288..1304) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1288..1304) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1406..1425) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1417..2226 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1484..1503) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2373..2961 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2862..2881 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3115..3132 /label=L4440 /note="L4440 vector, forward primer" terminator 3291..3318 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3410..3496 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3545..3567 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3559..3576 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3560..3576 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac 121 tttaagaagg agcccttcac catggacaac cttgggcata gagaaaatgg caggcaaagg 181 cctgagcaat ataaagcgct tcatactcag tggatgatac ctcaaaggca gctaaaagac 241 catcaaagta tgaatctcct ggcactgatg aatgagaagg acagtgccat ccgagaaagg 301 gaccatgctc tcgctgagaa gaaagcagct atagctgagc gagatatggc attcgcccag 361 cgggatgctg caatggctga acggaatgct gcaatagtgg agagggacaa cgccctagct 421 gcactcgaac tcgcccgtac aaatgggttt aatatgaata gtgggaatgg atttcaccaa 481 ggacctcctc tcaatggaac aaagaacatc catcaccatg accatctgtc tcatgtccaa 541 acatccccgc tgcagctggc caattctcca tatgaccatg tcagggagat gcacatatca 601 gaagcatacc ctatcgcaac agctcccgca agtgttggga aggctaagaa gccaaggaag 661 agcaattctc aagcttctcc atcgaagagg ccatcaggtg tactccgaaa aaccaagaaa 721 gcaaccagcg actggaagaa tgcggggact actggtggag caggagattc agcccgcgct 781 tctgtgatga agaacgagtg gaaggaccag gatctcggcc tgaaccaggt ggtgttcgac 841 gagtcaacca tgcccgcgcc tgcctgctca tgcacagggg agctccacca atgctacaaa 901 tggggcagtg gtgggtggca gtcgtcttgc tgcaccatga acatgtccat gcacccgctc 961 ccggtgatgc ccaacaggcg ccacgctcgc atggggggaa ggaagatgag cgggggcgcg 1021 ttcgcgaagc tgctcagccg gctggcagca gaaggtcatg acctctcgac tccggtggac 1081 ctcaaggacc actgggccaa gcatggcacg aaccggtaca tcaccatccg gaagggtggg 1141 cgcgccgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt 1201 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat 1261 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg 1321 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa 1381 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg 1441 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc 1501 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca 1561 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc 1621 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact 1681 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta 1741 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg 1801 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct 1861 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt 1921 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg 1981 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa 2041 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc 2101 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa 2161 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt 2221 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg 2281 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg 2341 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 2401 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 2461 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc 2521 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 2581 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 2641 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 2701 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 2761 gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 2821 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 2881 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 2941 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 3001 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 3061 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 3121 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg 3181 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 3241 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat 3301 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc 3361 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac 3421 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag 3481 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg 3541 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc c //