LOCUS Exported 3777 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1856-HQ858795 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3777) TITLE Maize TF ORFeome collection REFERENCE 2 (bases 1 to 3777) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3777 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" RBS 112..132 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(1334..1433) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1450..1469) /label=T7 /note="T7 promoter, forward primer" promoter complement(1451..1469) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1474..1490) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1474..1490) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1592..1611) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1603..2412 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1670..1689) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2559..3147 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3048..3067 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3301..3318 /label=L4440 /note="L4440 vector, forward primer" terminator 3477..3504 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3596..3682 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3731..3753 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3745..3762 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3746..3762 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac 121 tttaagaagg agcccttcac catggacgac gacgacgacg tgtcgccctc ctcctccccg 181 tcgccgccct cctcctccgc gctccccgtg gacggccccg tcaccgtcgc cgctgcgccg 241 ccgggcgctt ccatggcggt cgcgctcccg atccatagga ccgcggcgtc cctgtacgct 301 agcgccggcg gcgatggcgg cggcggcggc gggagggagg acgcctggag cgacggcgcc 361 acgtccgcgc tcatcgacgc ctggggcgag cgcttcgtcg cgctcggccg cggcagcctc 421 cgccacccgc agtggcagga ggtcgccgaa gccgtctcct cccgtgacgg ctactccaag 481 gcgcccaagt ccgatatcca gtgcaaaaac cgcatcgaca cgctcaagaa gaagtacaag 541 gtcgagagga tcaagccggt ctccggctgg cagttcttcg accgcctcga ttttctcctc 601 gcgcccacct acggcaacaa gcccggcaac ggcggcggcg ggcacaactc caacagccgc 661 agccagatgc ctgcggcgcc gcgagtgggc ttccctcaac gcagccgcac gccgctcatg 721 cccgcagcag gatccggggc caagcggagg gcgccttcgc cggagccgtc ggtgtcctcc 781 gattcctccg acggcttccc gccaatgccg gcccttccgg cggtgaacgg gaagaggaag 841 aggacggacg atgggcgctc cgacggaggc ggcgaccgcg cgcaggggct tcgggagctg 901 gctcaggcga tacggcgcct cggcgaggcg tacgagcgcg tggaggccac gaagctggaa 961 caggaggccg agatggagcg tcggcgcatg gacttcgcgc aggagctgga gtcgcagcgc 1021 gtgcggttct tcctcgacac gcagatggag ctcacgcagg ccaagaatca cgcgtctcct 1081 gccacggccg ccgtccctgc tgttggctcg tcgaggagga ttccgaggat gtcactggtt 1141 accgacgccg gtggcagcag caatcaccat agccgttacc ggatcagcca tgttgacagg 1201 caccgccatg ctccacgccc acattaccag cagtaccatg acaacaataa cgcgggggca 1261 gcggctgcaa gtgacggtga gcagtctgac gaggaagatt atgaagaaga gagccagaag 1321 ggtgggcgcg ccgacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta 1381 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca 1441 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg 1501 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca 1561 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg 1621 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg 1681 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat 1741 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag 1801 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc 1861 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag 1921 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg 1981 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt 2041 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac 2101 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc 2161 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag 2221 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat 2281 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt 2341 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat 2401 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact 2461 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac 2521 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 2581 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 2641 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 2701 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 2761 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 2821 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 2881 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 2941 cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 3001 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 3061 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 3121 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 3181 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 3241 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 3301 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 3361 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 3421 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa 3481 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc 3541 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg 3601 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg 3661 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca 3721 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccc //