LOCUS Exported 3801 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1180-HQ858802 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3801) TITLE Rice TF ORFeome collection REFERENCE 2 (bases 1 to 3801) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3801 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1358..1457) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1474..1493) /label=T7 /note="T7 promoter, forward primer" promoter complement(1475..1493) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1498..1514) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1498..1514) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1616..1635) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1627..2436 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1694..1713) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2583..3171 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3072..3091 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3325..3342 /label=L4440 /note="L4440 vector, forward primer" terminator 3501..3528 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3620..3706 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3755..3777 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3769..3786 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3770..3786 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc 121 atgggcgctc tttgtgattt ctgcggggag cagaggtcta tggtttattg ccgatcagat 181 gcagcatcct tgtgcttatc gtgtgaccgt aatgtgcatt cagctaatgc tctttctcgg 241 cgccatacaa ggacacttct gtgtgataga tgtgcttcac agcctgccat ggtccgctgc 301 cttgtagaaa atgcctcgct ttgccaaaat tgtgattgga atgggcatag tgctgggtct 361 tcagctgctg gtcataagag gcagaccata aactgctact cagggtgccc atcatcttca 421 gaactttcaa aaatctggac atttgtttcg gatattccta atgtagctcc tgagccgaat 481 tgcgagcaag ggataagcat gatgagcatc agcgacagtg gtgtcagtaa tcaagacaat 541 gctgcagggg acagcagttt gttagatatt gctagtgcaa cccttatgag tgatcttggc 601 actgctggca agccaaagtc tttgataggc tcctcatctg aagctggcgt gaaccttctt 661 ccacttgcta ctgatcagat ggctggatcc gtggattcga catcagctaa ggtgccctat 721 acggcagacc aagatatgtt cagcaaagac agtatatatg aagatttctg tgtggacgat 781 gttgatctta gtttcgaaaa ttatgaggaa ctatttggca cctcccatat tcaaacagag 841 caactgtttg atgatgctgg gatcgacagt tactttgaat cgaaggaaat accttctgga 901 aactctgatg agcccaagct gatgcagcca gtgaccagca atgcagtatc tgctgattca 961 gggatgtcga tcccaggagc caaaggtgat tccagtcttt gtatacctgt gaggcaagct 1021 aggtccagta tctctctttc attttctggt ttgactggtg agagcagtgc tggagattac 1081 caagattgtg gggtatcacc agtgctcctt atgggtgagc ctccttggca tcctcctggc 1141 cctgaggggt catttgctgg agctaccaga gacgatgcta tcacacgata caaggagaag 1201 aagaagagaa gaaaatttga caagaaaatc aggtatgcat ctcgcaaggc tagggcagat 1261 gtgagaaaaa gagtcaaggg ccggtttgtt aaggctggtg aagcatacga ctacgatcca 1321 ctctgtgaaa cgaggagcta caagggtggg cgcgccgacc cagctttctt gtacaaagtt 1381 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa 1441 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc 1501 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa 1561 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg 1621 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat 1681 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc 1741 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc 1801 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct 1861 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg 1921 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt 1981 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct 2041 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg 2101 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa 2161 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca 2221 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc 2281 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct 2341 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa 2401 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa 2461 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt 2521 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 2581 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 2641 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 2701 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac 2761 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 2821 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 2881 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 2941 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa 3001 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 3061 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 3121 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 3181 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 3241 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 3301 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 3361 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 3421 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag 3481 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat 3541 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt 3601 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga 3661 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc 3721 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg 3781 gccagtctta agctcgggcc c //