LOCUS Exported 3729 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1182-HQ858804 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3729) TITLE Rice TF ORFeome collection REFERENCE 2 (bases 1 to 3729) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3729 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1286..1385) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1402..1421) /label=T7 /note="T7 promoter, forward primer" promoter complement(1403..1421) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1426..1442) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1426..1442) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1544..1563) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1555..2364 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1622..1641) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2511..3099 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3000..3019 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3253..3270 /label=L4440 /note="L4440 vector, forward primer" terminator 3429..3456 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3548..3634 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3683..3705 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3697..3714 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3698..3714 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc 121 atggatggtg cttcctccat tagtcctgca tttacttcaa attatcagca aagtaagctg 181 gcagatccaa tccaatcaaa accgaagccc cttaccccaa aggatttaat gcctgtccaa 241 tttagttcgt cgctgcctga tcctacctca atctgttttg agggtatagc tgccataact 301 acacccaatt ttgaggatca tggcaacaga gccagcattg cttcaggggc aaaagatgtg 361 aacatgttcc caacatttaa agaccagtcc agtaatcgtc atgatgcact gttttctaat 421 gtcgatgggt acatgcaaca gagacgaaat aatcactttg gaactgaagt atgtggctta 481 tctgaaagca caggcatttc ccagtcggac cagttcacag tatcgaaatt ccagaatggc 541 aggcccagca gcttgggcct ttcgaatgga aatcttgggg ttctatcgac tgggagaaga 601 gaggaagcac gtgaaggctt gtttaggcct tgttctgtca agacaagtgc tgggaatgag 661 gagcagcaaa agcggcctgg tgatgtcaag ttgtttgggc aaattcttag ccatcaatca 721 tccttgcaaa gctctggttc atcagttcat ggaagcaaaa gcaagccccc ctcaccaaag 781 gttgataagc cagcttcttc taggttgttg agtaatccaa gggaacgcct ggtttattct 841 tccaggcctc caatcattgc taacttgggg ctagaggaga gagcgatgag gagttttgac 901 catatggatg ggcgaacaat acagcctgag cctatggtta tggtggcaaa gtgtcagaga 961 ccgtcagctg gtgtaccagt ttactcaacc aaaaatggcg cgctcagcgt gtttgcggaa 1021 tttcagcaac cttcaatgcc gccacataca tcagatcaca aactactaga aaactttgcc 1081 gatcttcata agaggaatgg aatcgaactc ctttcggggt tccagcagcc tgggaggctt 1141 gggggtgctg gggtcctggt tagcggtgta tctgaccctg tggctgctct caaggcacag 1201 tacggctctg gttcgaagat gttgagcagc agcaacgacg tggatacatg gaaagacata 1261 ggaagcagga agggtgggcg cgccgaccca gctttcttgt acaaagttgg cattataaga 1321 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt 1381 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc 1441 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata 1501 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc 1561 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat 1621 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat 1681 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat 1741 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc 1801 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa 1861 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg 1921 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat 1981 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt 2041 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa 2101 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt 2161 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac 2221 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag 2281 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat 2341 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc 2401 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac 2461 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 2521 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 2581 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 2641 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct 2701 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 2761 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 2821 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 2881 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg 2941 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 3001 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 3061 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 3121 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 3181 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 3241 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg 3301 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 3361 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta 3421 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt 3481 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc 3541 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg 3601 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg 3661 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag 3721 ctcgggccc //