LOCUS Exported 3432 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1187-HQ858809 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3432) TITLE Rice TF ORFeome collection REFERENCE 2 (bases 1 to 3432) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3432 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(989..1088) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1105..1124) /label=T7 /note="T7 promoter, forward primer" promoter complement(1106..1124) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1129..1145) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1129..1145) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1247..1266) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1258..2067 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1325..1344) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2214..2802 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2703..2722 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2956..2973 /label=L4440 /note="L4440 vector, forward primer" terminator 3132..3159 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3251..3337 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3386..3408 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3400..3417 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3401..3417 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc 121 atggaagcag acgcgagcca tacacccacc acctcctcct ccgtctccgt ctccttctcc 181 tcctcgtcgc tgtccacttc ctcctccacc tcctccctcg tcgacaatgg cgcgcaagac 241 cggcccaaga gctccaagcc caaacacgcc gccaagaagc gcaagagagc ggccgcggag 301 gaacctgcca atgccgccca cggcgcaggg gaggatacca gcagctgcag caccgacgac 361 aacgcggcgg cgagcggcaa ggcgcaggcg ggcggcggcg gcggcggcgt cgacagcagc 421 agcacctgca ccgccgcctc ggcgccgagg agcggcttca agcacccgtc gtaccgcggc 481 gtgcgccgcc ggagctgggg gaagtgggtg tcggagatcc gggagccccg caagaagtcg 541 cgcatctggc tgggtacctt ccccaccgcg gagatggcgg cgcgcgccca cgacgtggcc 601 gcgctcgcca tcaagggccg gaacgcgcac ctcaacttcc cggacagcgc ccacgagctg 661 ccccgcccgg agtccacctc cccggcagac atccaggccg ccgccgccaa ggctgccgcc 721 gaggtgcggt gcgaggagga gtcgtcgccg tcgtcgtcgc ccaccgccga gcaacccgag 781 gaggaagccg cctgccctga cacggtgcac gccgacggcg gccaggacaa tgctctcttc 841 gacctacccg accttcttct cgacctacgg gacgggctct ggtggtcgcc ggtgtggccg 901 gcggcactgg cggccgagga gtacgacggc ggcgacgccg tcgtgctcaa tgagccactc 961 ttgtgggccg agaagggtgg gcgcgccgac ccagctttct tgtacaaagt tggcattata 1021 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc 1081 attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt 1141 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat 1201 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg 1261 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta 1321 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg 1381 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat 1441 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc 1501 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga 1561 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg 1621 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc 1681 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg 1741 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat 1801 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac 1861 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca 1921 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta 1981 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt 2041 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag 2101 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt 2161 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 2221 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 2281 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 2341 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 2401 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 2461 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 2521 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 2581 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag 2641 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 2701 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 2761 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 2821 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 2881 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 2941 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 3001 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 3061 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt 3121 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag 3181 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg 3241 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa 3301 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc 3361 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt 3421 aagctcgggc cc //