LOCUS Exported 3387 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1236-HQ858819 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3387) TITLE Rice TF ORFeome collection REFERENCE 2 (bases 1 to 3387) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3387 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(944..1043) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1060..1079) /label=T7 /note="T7 promoter, forward primer" promoter complement(1061..1079) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1084..1100) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1084..1100) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1202..1221) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1213..2022 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1280..1299) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2169..2757 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2658..2677 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2911..2928 /label=L4440 /note="L4440 vector, forward primer" terminator 3087..3114 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3206..3292 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3341..3363 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3355..3372 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3356..3372 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc 121 atgaaggtgc agtgcgacgt gtgcgcggcc gaggcggcgt cggtgttctg ctgcgccgac 181 gaggccgcgc tgtgcgacgc gtgcgaccac cgggtgcacc gggccaacaa gctcgccggg 241 aagcaccgcc ggttctcgct gctcaacccc tcggcgtccg gccgctcgcc gacatcgacg 301 acggcgccgc tctgcgacat ctgccaggag aagaggggtt tcctgttctg caaggaggac 361 cgggcgatcc tgtgccgcga gtgcgacgtg ccggtgcaca cggcgagcga gctcaccatg 421 cgccacagcc ggtacctcct caccggcgtg cggctctcct cggagcctgc cgcgtccccg 481 gcgccgccgt cggaggagga gaacagcagc agcttctgct gcagcgccga cgacgccgtg 541 ccggccccgg cggcgcccgc cacgagccac ggcgggagca gcggcagcag cagcatctcc 601 gagtacctca ccacgctgcc cgggtggcac gtcgaggact tcctcgtcga cgacgccact 661 gccgaggccg ccgccgccgc cgccgccacc tcttccggca tctccgcgaa cgggccgtgt 721 cagggggtaa cacggatcgg agggctgcaa gaatccgccg gctaccctgc gtggatggcg 781 cagcagcagc tgtgctgcga cggcctcgtc gccggcgacg cgtcgccggc tagccgggag 841 cggtgggtgc cgcagatgta cgcggatcag cttgccgccg gcagcaagag atccaggacg 901 tccactgctt cttcctactc ctactggaag ggtgggcgcg ccgacccagc tttcttgtac 961 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc 1021 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac 1081 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt 1141 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat 1201 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac 1261 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg 1321 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa 1381 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt 1441 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc 1501 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa 1561 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat 1621 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac 1681 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc 1741 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat 1801 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga 1861 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag 1921 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg 1981 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg 2041 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa 2101 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa 2161 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 2221 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 2281 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 2341 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 2401 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 2461 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 2521 cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt 2581 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 2641 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 2701 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 2761 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 2821 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 2881 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 2941 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 3001 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct 3061 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag 3121 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca 3181 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac 3241 aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg 3301 cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa 3361 acgacggcca gtcttaagct cgggccc //