LOCUS Exported 942 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pAAV-minCMV-mCherry SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 942) AUTHORS Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P TITLE Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. JOURNAL Nat Biotechnol. 2011 Jan 19. ():. PUBMED 21248753 REFERENCE 2 (bases 1 to 942) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..942 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(65..775) /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(343..362) /label=mCherry-F /note="mCherry, forward primer" primer_bind 612..630 /label=mCherry-R /note="mCherry, reverse primer" regulatory 772..781 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(795..819) /label=LNCX /note="Human CMV promoter, forward primer" promoter complement(816..854) /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind complement(872..891) /label=T7 /note="T7 promoter, forward primer" promoter complement(873..891) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcga 61 attcttactt gtacagctcg tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt 121 actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccaacttg atgttgacgt 181 tgtaggcgcc gggcagctgc acgggcttct tggccttgta ggtggtcttg acctcagcgt 241 cgtagtggcc gccgtccttc agcttcagcc tctgcttgat ctcgcccttc agggcgccgt 301 cctcggggta catccgctcg gaggaggcct cccagcccat ggtcttcttc tgcattacgg 361 ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct 421 gcagggagga gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct 481 cccacttgaa gccctcgggg aaggacagct tcaagtagtc ggggatgtcg gcggggtgct 541 tcacgtaggc cttggagccg tacatgaact gaggggacag gatgtcccag gcgaagggca 601 gggggccacc cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct 661 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga 721 agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc accatggtgg 781 cggtacctcc aggcgatctg acggttcact aaacgagctc tgcttatata ggcctcccac 841 cgtacacgcc tacctcgaca tacgtggatc cccctatagt gagtcgtatt atctagaacg 901 cgtgcggccg caggaacccc tagtgatgga gttggccact cc //