LOCUS Exported 3738 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1347-HQ858837 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3738) TITLE Rice TF ORFeome collection REFERENCE 2 (bases 1 to 3738) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3738 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1295..1394) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1411..1430) /label=T7 /note="T7 promoter, forward primer" promoter complement(1412..1430) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1435..1451) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1435..1451) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1553..1572) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1564..2373 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1631..1650) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2520..3108 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3009..3028 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3262..3279 /label=L4440 /note="L4440 vector, forward primer" terminator 3438..3465 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3557..3643 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3692..3714 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3706..3723 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3707..3723 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc 121 atgatgatga tgggggaagg agtcagcagc gtcccgccgt ggtctcacct ccccgtgagc 181 ggagtcgatg tactcggcgg cggtggtggc ggtggggatg agatgacgcc gtacgtgatc 241 gccgcgctgc gggattacct gccggcgaat gatgtcgggg tgggggctga cgaggaggag 301 gaggccgcgg cgatggccgc ggcggtggac gcgtacgcgt gcgacgagtt ccggatgtac 361 gagttcaagg tgcggcggtg cgcgcgcggg cggagccatg actggaccga gtgccccttc 421 gcgcacccgg gggagaaggc gcgccgccgc gatccgcgca agtaccacta ctccggcacc 481 gcgtgcccgg acttccgcaa gggcgggtgc aagcgcggcg acgcctgcga gtacgcccac 541 ggcgtgtttg agtgttggct ccacccggcg cgctaccgca cccagccgtg caaggacggc 601 accgcctgcc gccgccgcgt ctgcttcttc gcccacaccc cggaccagct ccgcgtcctc 661 ccggcgcagc agtccagccc caggagcgtg gcgtcctcgc cgctggccga gtcctacgac 721 ggctcgccgc tgcgccgcca ggcgttcgag agctacctca ccaagaccat catgtcctcg 781 tccccgacca gcaccctcat gtctccgccc aagtcgcccc cgtcggagtc cccgccattg 841 tcgcccgacg gtgccgcggc catccgtcgc ggatcttggc ccggcgtcgg ctcgccggtg 901 aacgacgtcc tggcctcgtt ccgccagctc cgcctcaaca aggtgaagtc gtcgccgtcc 961 ggcgggtgga gctacccttc gtcgtccgcc gtctacgggt ctcccaaggc cgccaccggc 1021 ctctacagcc tccccaccac tccactggct tccacggcaa cggtgaccac cgcctccagc 1081 ttcatgccca acctggagcc actggacctc gggctcatcg gcgacgagga gccggtccag 1141 agggtggagt ccggaagagc cctccgggag aaggtgttcg agcgactgag ccgggatggt 1201 gccatctccg gcgacgccac agccttcgcc accgccggtg ttggcctcga cgttgattgg 1261 gtgtccgacc tcatcaacaa gggtgggcgc gccgacccag ctttcttgta caaagttggc 1321 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat 1381 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata 1441 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat 1501 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt 1561 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct 1621 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat 1681 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt 1741 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt 1801 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc 1861 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt 1921 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt 1981 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt 2041 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa 2101 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt 2161 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga 2221 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct 2281 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg 2341 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac 2401 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac 2461 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 2521 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 2581 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 2641 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 2701 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 2761 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 2821 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 2881 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg 2941 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 3001 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 3061 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 3121 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 3181 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 3241 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 3301 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 3361 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa 3421 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc 3481 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca 3541 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa 3601 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct 3661 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc 3721 agtcttaagc tcgggccc //