LOCUS Exported 3388 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1505-HQ858838 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3388) TITLE Rice TF ORFeome collection REFERENCE 2 (bases 1 to 3388) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3388 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(945..1044) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1061..1080) /label=T7 /note="T7 promoter, forward primer" promoter complement(1062..1080) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1085..1101) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1085..1101) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1203..1222) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1214..2023 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1281..1300) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2170..2758 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2659..2678 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2912..2929 /label=L4440 /note="L4440 vector, forward primer" terminator 3088..3115 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3207..3293 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3342..3364 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3356..3373 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3357..3373 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc 121 atgtcgagcg cgtcgtccat ggaagcgctc cacgccgcgg tgctcaagga ggagcagcag 181 cagcacgagg tggaggaggc gacggtcgtg acgagcagca gcgccacgag cggggaggag 241 ggcggacacc tgccgcaggg gtgggcgaag cggaagcggt cgcgccgcca gcgatcggag 301 gaggagaacc tcgcgctctg cctcctcatg ctcgcccgcg gcggccacca ccgcgtccag 361 gcgccgcctc cgctctcggc ttcggcgccc ccgccggcag gtgcggagtt caagtgctcc 421 gtctgcggca agtccttcag ctcctaccag gcgctcggcg gccacaagac gagccaccgg 481 gtcaagctgc cgactccgcc cgcagctccc gtcttggctc ccgcccccgt cgccgccttg 541 ctgccttccg ccgaggaccg cgagccagcc acgtcatcca ccgccgcgtc ctccgacggc 601 atgaccaaca gagtccacag gtgttccatc tgccagaagg agttccccac cgggcaggcg 661 ctcggcgggc acaagaggaa gcactacgac ggtggcgtag gcgccggcgc cggcgcatct 721 tcaaccgagc tcctggccac ggtggccgcc gagtccgagg tgggaagctc cggcaacggc 781 cagtccgcca cccgggcgtt cgacctcaac ctcccggccg tgccggagtt cgtgtggcgg 841 ccgtgctcca agggcaagaa gatgtgggac gaggaggagg gggtccagag ccccctcgcc 901 ttcaagaagc ccccggcttc tcaccgcgaa gggtgggcgc gccgacccag ctttcttgta 961 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat 1021 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta 1081 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat 1141 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa 1201 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa 1261 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc 1321 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa 1381 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt 1441 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac 1501 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga 1561 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa 1621 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa 1681 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt 1741 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga 1801 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg 1861 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga 1921 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat 1981 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg 2041 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa 2101 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa 2161 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 2221 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 2281 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 2341 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 2401 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 2461 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 2521 gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct 2581 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 2641 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 2701 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 2761 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 2821 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 2881 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 2941 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 3001 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 3061 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 3121 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 3181 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 3241 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 3301 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 3361 aacgacggcc agtcttaagc tcgggccc //