LOCUS Exported 1233 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.. ACCESSION . VERSION . KEYWORDS pET28a SnoopTag-MBP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1233) AUTHORS Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, Howarth M TITLE Programmable polyproteams built using twin peptide superglues. JOURNAL Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214. PUBMED 26787909 REFERENCE 2 (bases 1 to 1233) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1233 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 13..30 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 40..57 /codon_start=1 /product="thrombin recognition and cleavage site" /label=thrombin site /translation="LVPRGS" CDS 121..1218 /codon_start=1 /gene="malE (mutated)" /product="maltose binding protein from E. coli" /label=MBP /note="This version of the gene does not encode a signal sequence, so MBP will remain in the cytosol." /translation="KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW YAVRTAVINAASGRQTVDEALKDAQT" primer_bind 1192..1215 /label=MBP-F /note="Maltose binding protein, forward primer" ORIGIN 1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat 61 atgggaaaac tgggcgatat tgaatttatt aaagtgaaca aaggtagtgg tgaaagtggt 121 aaaatcgaag aaggtaaact ggtaatctgg attaacggcg ataaaggcta taacggtctc 181 gctgaagtcg gtaagaaatt cgagaaagat accggaatta aagtcaccgt tgagcatccg 241 gataaactgg aagagaaatt cccacaggtt gcggcaactg gcgatggccc tgacattatc 301 ttctgggcac acgaccgctt tggtggctac gctcaatctg gcctgttggc tgaaatcacc 361 ccggacaaag cgttccagga caagctgtat ccgtttacct gggatgccgt acgttacaac 421 ggcaagctga ttgcttaccc gatcgctgtt gaagcgttat cgctgattta taacaaagat 481 ctgctgccga acccgccaaa aacctgggaa gagatcccgg cgctggataa agaactgaaa 541 gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac cgtacttcac ctggccgctg 601 attgctgctg acgggggtta tgcgttcaag tatgaaaacg gcaagtacga cattaaagac 661 gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct tcctggttga cctgattaaa 721 aacaaacaca tgaatgcaga caccgattac tccatcgcag aagctgcctt taataaaggc 781 gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca acatcgacac cagcaaagtg 841 aattatggtg taacggtact gccgaccttc aagggtcaac catccaaacc gttcgttggc 901 gtgctgagcg caggtattaa cgccgccagt ccgaacaaag agctggcaaa agagttcctc 961 gaaaactatc tgctgactga tgaaggtctg gaagcggtta ataaagacaa accgctgggt 1021 gccgtagcgc tgaagtctta cgaggaagag ttggcgaaag atccacgtat tgccgccact 1081 atggaaaacg cccagaaagg tgaaatcatg ccgaacatcc cgcagatgtc cgctttctgg 1141 tatgccgtgc gtactgcggt gatcaacgcc gccagcggtc gtcagactgt cgatgaagcc 1201 ctgaaagacg cgcagactaa ttcgagctcg taa //