LOCUS       Exported                 474 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Bacterial expression of SpyCatcher with a peptide tag enabling 
            site-specific biotinylation..
ACCESSION   .
VERSION     .
KEYWORDS    AviTag-SpyCatcher
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, 
            Howarth M
  TITLE     Programmable polyproteams built using twin peptide superglues.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214.
  PUBMED    26787909
REFERENCE   2  (bases 1 to 474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             37..81
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             106..126
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac ggcagcggcc tgaacgatat ttttgaagcg
       61 cagaaaattg aatggcatga aggcgattac gacatcccaa cgaccgaaaa cctgtatttt
      121 cagggcgcca tggttgatac cttatcaggt ttatcaagtg agcaaggtca gtccggtgat
      181 atgacaattg aagaagatag tgctacccat attaaattct caaaacgtga tgaggacggc
      241 aaagagttag ctggtgcaac tatggagttg cgtgattcat ctggtaaaac tattagtaca
      301 tggatttcag atggacaagt gaaagatttc tacctgtatc caggaaaata tacatttgtc
      361 gaaaccgcag caccagacgg ttatgaggta gcaactgcta ttacctttac agttaatgag
      421 caaggtcagg ttactgtaaa tggcaaagca actaaaggtg acgctcatat ttaa
//
