LOCUS Exported 2661 bp ds-DNA linear SYN 25-MAY-2021 DEFINITION Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.. ACCESSION . VERSION . KEYWORDS pET21 MBPx-SpyCatcher SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2661) AUTHORS Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, Howarth M TITLE Programmable polyproteams built using twin peptide superglues. JOURNAL Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214. PUBMED 26787909 REFERENCE 2 (bases 1 to 2661) AUTHORS . TITLE Direct Submission JOURNAL Exported May 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2661 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 13..30 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 40..57 /codon_start=1 /product="thrombin recognition and cleavage site" /label=thrombin site /translation="LVPRGS" ORIGIN 1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat 61 atggctagca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc 121 tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc 181 gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc 241 cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg 301 gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc 361 gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt 421 tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat 481 aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc 541 acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatggcga cattaaagac 601 gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct tcctggttga cctgattaaa 661 aacaaacaca tgaatgcaga caccgattac tccatcgcag aagctgcctt taataaaggc 721 gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca acatcgacac cagcaaagtg 781 aattatggtg taacggtact gccgaccttc aagggtcaac catccaaacc gttcgttggc 841 gtgctgagcg caggtattaa cgccgccagt ccgaacaaag agctggcaaa agagttcctc 901 gaaaactatc tgctgactga tgaaggtctg gaagcggtta ataaagacaa accgctgggt 961 gccgtagcgc tgaagtctta cgaggaagag ttggtgaaag atccacgtgt ggccgccact 1021 atggaaaacg cccagaaagg tgaaatcatg ccgaacatcc cgcagatgtc cgctttctgg 1081 tatgccgtgc gtactgcggt gatcaacgcc gccagcggtc gtcagactgt cgatgaagcc 1141 ctgaaagacg cgcagacgaa ttctagttcc ggcggatccg gaggtggatc cggaaagata 1201 gaggagggta aactggtaat ctggattaac ggcgataaag gctataacgg tctcgctgaa 1261 gtcggtaaga aattcgagaa agataccgga attaaagtca ccgttgagca tccggataaa 1321 ctggaagaga aattcccaca ggttgcggca actggcgatg gccctgacat tatcttctgg 1381 gcacacgacc gctttggtgg ctacgctcaa tctggcctgt tggctgaaat caccccggac 1441 aaagcgttcc aggacaagct gtatccgttt acctgggatg ccgtacgtta caacggcaag 1501 ctgattgctt acccgatcgc tgttgaagcg ttatcgctga tttataacaa agatctgctg 1561 ccgaacccgc caaaaacctg ggaagagatc ccggcgctgg ataaagaact gaaagcgaaa 1621 ggtaagagcg cgctgatgtt caacctgcaa gaaccgtact tcacctggcc gctgattgct 1681 gctgacgggg gttatgcgtt caagtatggc gacattaaag acgtgggcgt ggataacgct 1741 ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca 1801 gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc 1861 aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta 1921 ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt 1981 aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact 2041 gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct 2101 tacgaggaag agttggtgaa agatccacgt gtggccgcca ctatggaaaa cgcccagaaa 2161 ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg 2221 gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact 2281 aattcgagct cgggttcggg cggtagtggt gccatggttg ataccttatc aggtttatca 2341 agtgagcaag gtcagtccgg tgatatgaca attgaagaag atagtgctac ccatattaaa 2401 ttctcaaaac gtgatgagga cggcaaagag ttagctggtg caactatgga gttgcgtgat 2461 tcatctggta aaactattag tacatggatt tcagatggac aagtgaaaga tttctacctg 2521 tatccaggaa aatatacatt tgtcgaaacc gcagcaccag acggttatga ggtagcaact 2581 gctattacct ttacagttaa tgagcaaggt caggttactg taaatggcaa agcaactaaa 2641 ggtgacgctc atatttaata g //