LOCUS Exported 3396 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1610-HQ858847 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3396) TITLE Rice TF ORFeome collection REFERENCE 2 (bases 1 to 3396) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3396 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" RBS 112..132 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(953..1052) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1069..1088) /label=T7 /note="T7 promoter, forward primer" promoter complement(1070..1088) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1093..1109) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1093..1109) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1211..1230) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1222..2031 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1289..1308) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2178..2766 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2667..2686 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2920..2937 /label=L4440 /note="L4440 vector, forward primer" terminator 3096..3123 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3215..3301 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3350..3372 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3364..3381 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3365..3381 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac 121 tttaagaagg agcccttcac catggacccc gcccccacgc tcgccgccga gctctggcga 181 acgccgtacc tcggtggcgg tggtggtgga ggaggaggag ggcgtgggtt ggaggcggcg 241 gcgtccgggg tcacggagca gagcaatggg agccgcgggg gtggcggagg aggaggcgca 301 gggcggagga ggcagcggga ggcccccgcg ctcgaggacg actcgtccag gatcgtctcc 361 gcttccggag gaggaggcgg cggtcaggat ttgactgatt cagaagcaaa gcgtttcaag 421 gcaagtaaat caagtggaga taatagcagt ttaagaacag aggctgaaac tgattcaaga 481 aatgctagca agtctggtga tcaaaatccg ccaccaccag aaccaccaaa gcaagactac 541 atccatgtga gggcaagaag gggtcaagct actgacagcc atagccttgc cgaaagggca 601 cgtcgtgaga aaataagtga gcggatgaaa attctccagg atcttgtccc tggatgcaac 661 aaggttattg ggaaagcatc agtacttgat gagataatta attatatcca ggctttgcaa 721 cggcaagttg agtttttgtc catgaagctt gaggctgtta atgctcatgt gaataatgga 781 atcgaagcat ttccacccaa agattttggt gctcaggtat acaacacggc tcctggattg 841 acgtttgatc cacaaacacc aagagaatac gcccagggat caacaccatc ggaatggctc 901 cacatgcaga ttggtggcac ctatgaaaga gtgacaaagg gtgggcgcgc cgacccagct 961 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag 1021 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag 1081 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga 1141 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata 1201 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta 1261 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa 1321 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa 1381 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg 1441 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg 1501 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat 1561 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct 1621 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga 1681 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt 1741 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact 1801 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt 1861 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc 1921 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat 1981 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg 2041 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca 2101 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa 2161 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 2221 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 2281 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg 2341 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 2401 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 2461 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 2521 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc 2581 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 2641 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 2701 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 2761 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 2821 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 2881 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 2941 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 3001 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg 3061 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct 3121 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc 3181 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca 3241 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt 3301 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga 3361 cgttgtaaaa cgacggccag tcttaagctc gggccc //