LOCUS Exported 3618 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1616-HQ858853 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3618) TITLE Rice TF ORFeome collection REFERENCE 2 (bases 1 to 3618) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3618 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" RBS 112..132 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" regulatory 305..314 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" protein_bind complement(1175..1274) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1291..1310) /label=T7 /note="T7 promoter, forward primer" promoter complement(1292..1310) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1315..1331) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1315..1331) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1433..1452) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1444..2253 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1511..1530) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2400..2988 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2889..2908 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3142..3159 /label=L4440 /note="L4440 vector, forward primer" terminator 3318..3345 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3437..3523 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3572..3594 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3586..3603 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3587..3603 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac 121 tttaagaagg agcccttcac catggagagc ttgtgtgggc agcttgagca tgaacatgag 181 cagcagctgc accaatcctc ctcatcttca cttctcccct actgcttcct ttctgctcca 241 gagcctcttc ctgtcccagg cttccctgcc ggctgcagca accttgaaga gaaggcagca 301 gcagccatgg cggcctatga gtatgagagc agcagctgca gctcccttga ccccacctca 361 atgccaatgg tgtactcacc aatagtgctg cagccacagg agtgccctct ttcctttgtc 421 tttgacaatg ccgctgccgc tgccggcgac aacaaatggg tgcctggcat tcaaggaagt 481 tgcccttgtt cattgggctc aactcaagat atggatgcaa gttggggcaa gagtagaaag 541 cacaagagga gcaatgtggg cttgaaggga cttgaggaga aaaaggcaag aagggtggta 601 ctacatcaac atgatgatga tgtgaagaag aaggccaagg aggcagcagg tggggagcca 661 ccagctgggt acattcatgt gagggcaagg agaggccaag caacagacag ccacagcctt 721 gctgagaggg tgagaaggga gaagatcagt gagaggatga agatgctgca aagcctggtt 781 cctggttgtg acaaggtcac agggaaagcc ctcatgctag atgagatcat cagctatgtg 841 caatctctgc agaaccaagt tgagtttctg tccatgaagc ttgcctccct tagccctttg 901 atgtatgagt ttggaccggg cattgatatg cacccagatg ttcttcgaca gcttgcaaag 961 atgccacatg agatggtgca gtgcatgggc caaatgggga gtacaggcat ttcactccaa 1021 ggactaggag gaggacccac aggctttgct caggatggca gcagccacat taacatggtg 1081 gtgatgcaag ttggagagca aggccagcag cagggctctc ttcaccaggt cgagatgagc 1141 agccactgct tctttcataa gggtgggcgc gccgacccag ctttcttgta caaagttggc 1201 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat 1261 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata 1321 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat 1381 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt 1441 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct 1501 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat 1561 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt 1621 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt 1681 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc 1741 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt 1801 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt 1861 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt 1921 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa 1981 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt 2041 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga 2101 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct 2161 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg 2221 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac 2281 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac 2341 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 2401 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 2461 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 2521 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 2581 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 2641 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 2701 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 2761 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg 2821 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 2881 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 2941 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 3001 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 3061 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 3121 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 3181 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 3241 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa 3301 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc 3361 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca 3421 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa 3481 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct 3541 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc 3601 agtcttaagc tcgggccc //