LOCUS Exported 4044 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUT1673-HQ858864 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4044) TITLE Rice TF ORFeome collection REFERENCE 2 (bases 1 to 4044) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4044 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" RBS 112..132 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(1601..1700) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1717..1736) /label=T7 /note="T7 promoter, forward primer" promoter complement(1718..1736) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1741..1757) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1741..1757) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1859..1878) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1870..2679 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1937..1956) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2826..3414 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3315..3334 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3568..3585 /label=L4440 /note="L4440 vector, forward primer" terminator 3744..3771 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3863..3949 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3998..4020 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 4012..4029 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 4013..4029 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac 121 tttaagaagg agcccttcac catgaactgc gggccgcccg accagttgcc gccggcgacg 181 gcgccgtcgt gcttcctcaa cctcaactgg gaccagtcca tggacgcggc ggcgggaggg 241 cacctcgacc cggcgctcag ctccatggtg tcctcgccgg cgtccaactc gacgggcgct 301 ctccacggga tctcgccgca gccgcactac ggtggcggga cgccgctcag ctcgcccccc 361 aagctcaacc tgtccatgat ggggcagttc caccactacg ccgcgccgcc gcaggtgggc 421 ggcggcggag gcggaggcgg gggcctgcca atcctcgaga acctgatgcc catgggccac 481 ctcgaccagt tcctcgccga ccctggcttc gccgagcgcg ccgcgaggct ctccggcttc 541 gacgcccgcg gcggcggcgg aggaggaggc tacggcggcg ccggcccggc gcaattcggc 601 ctccctgacg ccggcgcggc cggcgcatcg aaggagatgg agctcgggaa cacccgggac 661 gagtcgtcgg tgtctgatcc ggcgcccggc ggcgccgaga ttccgcccaa gggggcttcc 721 gacggcaatg cacggaagcg gaaggcctcc gggaagggca aaggcaagga cagccccatg 781 tccacctccg ccgccaagga ggattccagc ggcaagcgtt gcaaatcgac ggaggagagc 841 aatgcggccg ccgaggagaa ttccggcaag gggaaggccg cgcagagcaa cagcgagaat 901 ggcggcggca agaagcaagg gaaggacagc tcgtcgaagc cccccgagcc gcccaaggac 961 tacatccatg tccgcgcccg gcgcggcgag gcgacggaca gccacagcct cgccgagagg 1021 gtgagaagag agaagataag ccagaggatg aagctgctgc aggatctcgt gccgggttgt 1081 aacaaggtgg ttggaaaggc tgtcatgctc gatgaaatca taaactatgt gcagtcgttg 1141 caacggcaag tcgagttttt gtccatgaag ttggccactg tgaatcccca gctggacttc 1201 aacaatttgc ctaacctcct tgctaaagat atgcaccagt catgcagccc gttacagagc 1261 tcacattttc cactagagac ctcaggtgcg ccgctgccct acattaacca gcctcagcaa 1321 gggaaccctc tcggttgtgg cctgacgaac ggcatggaca accagggttc tatgcaccca 1381 ttggacccgg cattttgccg gccaatgggc tcgcaccatc ctttcctcaa tggggttagc 1441 gatgcggctt ctcaggttgg tgctttctgg caagatgatc tccaaagtgt tgttcagatg 1501 gatatggggc aaagtcagga gatcgccacc tcttccaata gctacaatgg atcgttgcaa 1561 acagtccaca tgaaaatgga gcttaagggt gggcgcgccg acccagcttt cttgtacaaa 1621 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt 1681 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg 1741 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca 1801 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca 1861 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg 1921 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca 1981 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt 2041 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg 2101 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact 2161 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat 2221 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt 2281 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt 2341 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg 2401 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc 2461 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga 2521 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt 2581 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat 2641 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg 2701 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc 2761 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 2821 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 2881 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 2941 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 3001 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 3061 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 3121 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 3181 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc 3241 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 3301 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 3361 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 3421 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 3481 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 3541 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 3601 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 3661 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc 3721 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt 3781 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa 3841 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac 3901 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag 3961 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg 4021 acggccagtc ttaagctcgg gccc //