LOCUS Exported 9613 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Express Luc2 and copGFP. ACCESSION . VERSION . KEYWORDS pCDH-EF1-Luc2-P2A-copGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9613) TITLE express gene of interest under truncated EF-1 promoter JOURNAL Unpublished REFERENCE 2 (bases 1 to 9613) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9613 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 234..414 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 458..583 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1076..1309 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1493..1537 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 1804..1921 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2013..3191 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2244..3182 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3139..3159 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" CDS 3207..4856 /codon_start=1 /product="firefly luciferase" /label=luciferase /note="synthetic luc2 version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" CDS 4872..4928 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 4953..5615 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /label=CopGFP /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" misc_feature 5708..6296 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5761..5781) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6179..6190) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6370..6603 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 6675..6796 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6712..6731) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6766..6785 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 6815..6950 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6877..6896 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(6988..7004) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6988..7004) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7001..7023) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7012..7028 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7036..7066) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7081..7102 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7219..7236) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7390..7978) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7470..7489) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8149..9009) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8772..8791 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9010..9114) /gene="bla" /label=AmpR promoter primer_bind 9182..9200 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(9238..9260) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 9360..9379 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 9573..9595 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 9587..9604 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 9588..9604 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat 1981 ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc 2041 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct 2101 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc 2161 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca 2221 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct 2281 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc 2341 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag 2401 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa 2461 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt 2521 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag 2581 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc 2641 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt 2701 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc 2761 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc 2821 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg 2881 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca 2941 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt 3001 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga 3061 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt 3121 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc 3181 aggtgtcgtg agcaattgga tccaccatgg aagatgccaa aaacattaag aagggcccag 3241 cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc 3301 gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta 3361 cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc 3421 tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg 3481 tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc 3541 gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag 3601 ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca 3661 tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt 3721 tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca 3781 tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc 3841 accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca 3901 tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca 3961 cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc 4021 tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat 4081 ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga 4141 tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc 4201 acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca 4261 cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta 4321 aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg 4381 tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca 4441 tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact 4501 tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag 4561 ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc 4621 tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa 4681 ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc 4741 tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg 4801 cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgaagc 4861 ttggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc 4921 ctggacctat ggagagcgac gagagcggcc tgcccgccat ggagatcgag tgccgcatca 4981 ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc acccccaagc 5041 agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc agcccctacc 5101 tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc agcggctacg 5161 agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc atcgagaagt 5221 acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga 5281 tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc ttcaccgaca 5341 agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat aacgtgctgg 5401 tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg 5461 acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac gggggcccca 5521 tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc atcgtggagt 5581 accagcacgc cttcaagacc cccattgcct tcgccagatc ccgcgctcag tcgtccaatt 5641 ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgctaaggc gcgccgaatt 5701 cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 5761 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 5821 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 5881 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 5941 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 6001 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 6061 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg 6121 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 6181 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 6241 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg 6301 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 6361 aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta 6421 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 6481 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 6541 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 6601 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 6661 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 6721 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 6781 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc 6841 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 6901 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg 6961 cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 7021 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 7081 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 7141 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 7201 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 7261 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 7321 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 7381 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 7441 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 7501 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 7561 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 7621 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 7681 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 7741 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 7801 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 7861 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 7921 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 7981 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 8041 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 8101 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 8161 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 8221 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 8281 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 8341 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 8401 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 8461 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 8521 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 8581 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 8641 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 8701 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 8761 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 8821 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 8881 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 8941 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 9001 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 9061 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 9121 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 9181 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 9241 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 9301 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 9361 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 9421 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 9481 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 9541 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 9601 cagtgccaag ctg //