LOCUS       Exported                 750 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  flySTARR-seq validation vector with Gateway cassette upstream of the
            firefly luciferase gene and the DSCP core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pGL3-Gateway-DSCP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Arnold CD, Gerlach D, Stelzer C, Boryn LM, Rath M, Stark A
  TITLE     Genome-wide quantitative enhancer activity maps identified by 
            STARR-seq.
  JOURNAL   Science. 2013 Mar 1;339(6123):1074-7. doi: 10.1126/science.1232542. 
            Epub 2013 Jan 17.
  PUBMED    23328393
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    131..252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(142..161)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     196..215
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(500..517)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag
       61 gccaagaagg gcggaaagat cgccgtgtaa ttctagagtc ggggcggccg gccgcttcga
      121 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
      181 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
      241 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
      301 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg
      361 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
      421 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
      481 ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      541 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      601 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      661 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      721 gtcagaggtg gcgaaacccg acaggactat
//
