LOCUS       Exported                1026 bp ds-DNA     linear   SYN 09-JUL-2021
DEFINITION  Expresses 3xFNLDD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    3xFNLDD/pCMV-7.1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Fujita T, Kitaura F, Fujii H
  TITLE     A Critical Role of the Thy28-MYH9 Axis in B Cell-Specific Expression
            of the Pax5 Gene in Chicken B Cells.
  JOURNAL   PLoS One. 2015 Jan 21;10(1):e0116579. doi: 
            10.1371/journal.pone.0116579. eCollection 2015.
  PUBMED    25607658
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 9, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     12..31
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12..30
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(69..398)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      83..218
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(137..156)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     383..403
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     831..850
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 gattacgaat ttaatacgac tcactatagg gaattaactt tctggttttt cagttcctcg
       61 aggagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc
      121 tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
      181 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
      241 atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
      301 gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
      361 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagcca agctggccgc
      421 gtacccaatt caacaggcat ctactgagtg gacccaacgc atgagaggac agtgccaagc
      481 aagcaactca aatgtcccac cggttgggca tggccaggta gcctatgctg tgtctggacg
      541 tcctcctgct ggtatagtta ttttaaaatc agaaggacag ggaagggagc agtggttcac
      601 gcctgtaatc ccagcaattt gggaggccaa ggtgggtaga tcacctgaga ttaggagttg
      661 gagaccagcc tggccaatat ggtgaaaccc cgtctctacc aaaaaaacaa aaattagctg
      721 agcctggtca tgcatgcctg gaatcccaac aactcgggag gctgaggcag gagaatcgct
      781 tgaacccagg aggcggagat tgcagtgagc caagattgtg ccactgcact ccagcttggt
      841 tcccaataga ccccgcaggc cctacaggtt gtcttcccaa cttgcccctt gctccatacc
      901 acccccctcc accccataat attatagaag gacacctagt cagacaaaat gatgcaactt
      961 aattttatta ggacaaggct ggtgggcact ggagtggcaa cttccagggc caggagaggc
     1021 actggg
//
