LOCUS Exported 5367 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS 811 pSG5L HA PTEN C124S SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5367) AUTHORS Ramaswamy S, Nakamura N, Vazquez F, Batt DB, Perera S, Roberts TM, Sellers WR TITLE Regulation of G1 progression by the PTEN tumor suppressor protein is linked to inhibition of the phosphatidylinositol 3-kinase/Akt pathway. JOURNAL Proc Natl Acad Sci U S A. 1999 Mar 2. 96(5):2110-5. PUBMED 10051603 REFERENCE 2 (bases 1 to 5367) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5367 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1..27 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" polyA_signal 1301..1422 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1338..1357) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1392..1411 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(1456..1473) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1627..2215) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1707..1726) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2386..3246) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3009..3028 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3247..3351) /gene="bla" /label=AmpR promoter primer_bind 3419..3437 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(3475..3497) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 3597..3616 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" rep_origin complement(3683..4138) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3820..3841) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4032..4051 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4264..4286 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 4278..4295 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 4279..4295 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4332..4352) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4440..4650 /label=SV40 promoter /note="SV40 early promoter" promoter 4462..4658 /label=SV40 promoter /note="SV40 early promoter" rep_origin 4509..4644 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4571..4590 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 4689..5261 /label=-beta-globin intron /note="intron from rabbit beta-globin gene" primer_bind 5269..5288 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" primer_bind 5316..5335 /label=T7 /note="T7 promoter, forward primer" promoter 5316..5334 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 tacccctacg acgtgcccga ctacgcctcc ctcggatcca tgacagccat catcaaagag 61 atcgttagca gaaacaaaag gagatatcaa gaggatggat tcgacttaga cttgacctat 121 atttatccaa acattattgc tatgggattt cctgcagaaa gacttgaagg cgtatacagg 181 aacaatattg atgatgtagt aaggtttttg gattcaaagc ataaaaacca ttacaagata 241 tacaatcttt gtgctgaaag acattatgac accgccaaat ttaattgcag agttgcacaa 301 tatccttttg aagaccataa cccaccacag ctagaactta tcaaaccctt ttgtgaagat 361 cttgaccaat ggctaagtga agatgacaat catgttgcag caattcactc caaggctgga 421 aagggacgaa ctggtgtaat gatatgtgca tatttattac atcggggcaa atttttaaag 481 gcacaagagg ccctagattt ctatggggaa gtaaggacca gagacaaaaa gggagtaact 541 attcccagtc agaggcgcta tgtgtattat tatagctacc tgttaaagaa tcatctggat 601 tatagaccag tggcactgtt gtttcacaag atgatgtttg aaactattcc aatgttcagt 661 ggcggaactt gcaatcctca gtttgtggtc tgccagctaa aggtgaagat atattcctcc 721 aattcaggac ccacacgacg ggaagacaag ttcatgtact ttgagttccc tcagccgtta 781 cctgtgtgtg gtgatatcaa agtagagttc ttccacaaac agaacaagat gctaaaaaag 841 gacaaaatgt ttcacttttg ggtaaataca ttcttcatac caggaccaga ggaaacctca 901 gaaaaagtag aaaatggaag tctatgtgat caagaaatcg atagcatttg cagtatagag 961 cgtgcagata atgacaagga atatctagta cttactttaa caaaaaatga tcttgacaaa 1021 gcaaataaag acaaagccaa ccgatacttt tctccaaatt ttaaggtgaa gctgtacttc 1081 acaaaaacag tagaggagcc gtcaaatcca gaggctagca gttcaacttc tgtaacacca 1141 gatgttagtg acaatgaacc tgatcattat agatattctg acaccactga ctctgatcca 1201 gagaatgaac cttttgatga agatcagcat acacaaatta caaaagtctg agaattcccc 1261 gatatcgcct cgagtctaga tccagatctt attaaagcag aacttgttta ttgcagctta 1321 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 1381 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggtcgactct 1441 agactcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 1501 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 1561 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 1621 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 1681 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 1741 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 1801 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 1861 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 1921 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 1981 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 2041 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 2101 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 2161 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 2221 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 2281 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 2341 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 2401 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 2461 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 2521 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 2581 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 2641 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 2701 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 2761 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 2821 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 2881 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 2941 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 3001 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 3061 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 3121 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 3181 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 3241 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 3301 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 3361 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 3421 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 3481 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 3541 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 3601 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 3661 aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta 3721 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 3781 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca 3841 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 3901 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta 3961 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg 4021 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg 4081 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc 4141 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 4201 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 4261 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtcgacttct gaggcggaaa 4321 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 4381 cagaagtatg caaagcatgc atctcaatta gtcagcaagg aaagtcccca ggctccccag 4441 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 4501 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 4561 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 4621 agtgaagagg cttttttgga ggcctaggct tttgcaaaaa gctccggatc gatcctgaga 4681 acttcagggt gagtttgggg acccttgatt gttctttctt tttcgctatt gtaaaattca 4741 tgttatatgg agggggcaaa gttttcaggg tgttgtttag aatgggaaga tgtcccttgt 4801 atcaccatgg accctcatga taattttgtt tctttcactt tctactctgt tgacaaccat 4861 tgtctcctct tattttcttt tcattttctg taactttttc gttaaacttt agcttgcatt 4921 tgtaacgaat ttttaaattc acttttgttt atttgtcaga ttgtaagtac tttctctaat 4981 cacttttttt tcaaggcaat cagggtatat tatattgtac ttcagcacag ttttagagaa 5041 caattgttat aattaaatga taaggtagaa tatttctgca tataaattct ggctggcgtg 5101 gaaatattct tattggtaga aacaactaca tcctggtcat catcctgcct ttctctttat 5161 ggttacaatg atatacactg tttgagatga ggataaaata ctctgagtcc aaaccgggcc 5221 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg 5281 ttattgtgct gtctcatcat tttggcaaag aattgtaata cgactcacta tagggcgaat 5341 tgtcgacccg ggaagcttac catggcc //