LOCUS Exported 9430 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expresses two gRNAs targeting the TFRC promoter. ACCESSION . VERSION . KEYWORDS pDECKO_TFRC_C (with mCherry) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9430) AUTHORS Aparicio-Prat E, Arnan C, Sala I, Bosch N, Guigo R, Johnson R TITLE DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs. JOURNAL BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z. PUBMED 26493208 REFERENCE 2 (bases 1 to 9430) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9430 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 87..1264 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 318..1255 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1212..1232 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" CDS 1277..1873 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(1277..1296) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 1773..1793 /label=Puro-F /note="Puromycin resistance gene, forward primer" CDS 1883..1936 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" regulatory 1943..1952 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1949..2659 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(2094..2112) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 2362..2381 /label=mCherry-F /note="mCherry, forward primer" misc_feature 2675..3263 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2728..2748) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3146..3157) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 3335..3568 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 3646..3767 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3683..3702) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3737..3756 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 3807..3942 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3869..3888 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(3962..3981) /label=T7 /note="T7 promoter, forward primer" promoter complement(3963..3981) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3991..4008) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3991..4007) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4000..4022) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 4149..4604 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4236..4255) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4446..4467 /label=F1ori-F /note="F1 origin, forward primer" promoter 4630..4734 /gene="bla" /label=AmpR promoter CDS 4735..5595 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4953..4972) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5766..6354 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6255..6274 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6508..6525 /label=L4440 /note="L4440 vector, forward primer" protein_bind 6642..6663 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6678..6708 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6716..6732 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6721..6743 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 6740..6756 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 6740..6756 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 6775..6795 /label=T3 /note="T3 promoter, forward primer" promoter 6777..6795 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 6823..7049 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 7050..7230 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 7277..7402 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 7895..8128 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 8313..8357 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 8535..8775 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 8535..8555 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 8706..8725 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 8805..8880 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" promoter 8887..9101 /label=H1 promoter /note="human H1 RNA promoter" primer_bind 9022..9041 /label=H1 /note="Human H1 promoter, forward primer" misc_RNA 9130..9205 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" misc_feature 9261..9378 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct 61 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat 121 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa 181 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg 241 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt 301 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg 361 gttatggccc ttgcgtgcct tgaattactt ccactggctg cagtacgtga ttcttgatcc 421 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc 481 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg 541 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg 601 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca 661 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac 721 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca 781 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc 841 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc 901 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc 961 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta 1021 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg 1081 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt 1141 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc 1201 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc 1261 gtgacgtacg gccaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga 1321 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca 1381 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac 1441 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt 1501 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat 1561 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc 1621 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggagtctcgc ccgaccacca 1681 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg 1741 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg 1801 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg 1861 caagcccggt gccggatcgg gagagggcag aggaagtctg ctaacatgcg gtgacgtcga 1921 ggagaatcct ggcccaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc 1981 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga 2041 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct 2101 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat 2161 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc 2221 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac 2281 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg 2341 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc 2401 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa 2461 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc 2521 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga 2581 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat 2641 ggacgagctg tacaagtaaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg 2701 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 2761 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 2821 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 2881 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 2941 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 3001 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 3061 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg 3121 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 3181 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 3241 ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta 3301 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga 3361 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 3421 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 3481 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 3541 ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt 3601 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca 3661 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 3721 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc 3781 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 3841 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 3901 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc 3961 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg 4021 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg 4081 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg 4141 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 4201 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 4261 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 4321 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 4381 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 4441 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 4501 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 4561 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg 4621 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 4681 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 4741 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 4801 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 4861 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 4921 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 4981 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 5041 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 5101 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 5161 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 5221 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 5281 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 5341 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 5401 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 5461 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 5521 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 5581 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 5641 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 5701 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 5761 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 5821 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 5881 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 5941 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 6001 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 6061 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 6121 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 6181 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 6241 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 6301 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 6361 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 6421 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 6481 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 6541 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 6601 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 6661 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 6721 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt 6781 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac 6841 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa 6901 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc 6961 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg 7021 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct 7081 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 7141 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 7201 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa 7261 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag 7321 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga 7381 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa 7441 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag 7501 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag 7561 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt 7621 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa 7681 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc 7741 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat 7801 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa 7861 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg 7921 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat 7981 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc 8041 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg 8101 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt 8161 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga 8221 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact 8281 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag 8341 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat 8401 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta 8461 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc 8521 cgaggggacc cagagagggc ctatttccca tgattccttc atatttgcat atacgataca 8581 aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa 8641 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta 8701 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttataca 8761 tcttgtggaa aggacgaaac accgggggag cgggaaagcg gtcggtttta gagctagaaa 8821 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc 8881 ttttttgaac gctgacgtca tcaacccgct ccaaggaatc gcgggcccag tgtcactagg 8941 cgggaacacc cagcgcgcgt gcgccctggc aggaagatgg ctgtgaggga caggggagtg 9001 gcgccctgca atatttgcat gtcgctatgt gttctgggaa atcaccataa acgtgaaatg 9061 tctttggatt tgggagtctt ataagttctg tatgagacca ctctttccca actgaccttc 9121 aggcccgtag ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact 9181 tgaaaaagtg gcaccgagtc ggtgcttttt taagcttggc gtaactagat cttgagacaa 9241 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg 9301 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt 9361 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg 9421 ctcgaggggg //