LOCUS Exported 5425 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Luciferase reporter. ACCESSION . VERSION . KEYWORDS pSGE_768_Nhe2-EcR_dCP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5425) AUTHORS Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A TITLE Transcriptional regulators form diverse groups with context-dependent regulatory functions. JOURNAL Nature. 2015 Nov 9. doi: 10.1038/nature15545. PUBMED 26550828 REFERENCE 2 (bases 1 to 5425) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5425 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 39..160 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(50..69) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 104..123 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(408..425) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(579..1167) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(659..678) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1338..2198) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1961..1980 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2199..2303) /gene="bla" /label=AmpR promoter rep_origin 2330..2785 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2417..2436) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2627..2648 /label=F1ori-F /note="F1 origin, forward primer" polyA_signal 2916..2964 /note="synthetic polyadenylation signal" misc_feature 2978..3069 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" primer_bind 3018..3037 /label=RVprimer3 /note="pGL3 vector, forward primer" protein_bind 3083..3107 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(3550..3574) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 3765..3774 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3771..5423 /codon_start=1 /gene="luc+" /product="firefly luciferase" /label=luciferase /note="enhanced luc+ version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" primer_bind complement(3837..3855) /label=LucNrev /note="Firefly luciferase, reverse primer" ORIGIN 1 ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg 61 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat 121 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca 181 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta 241 caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag 301 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct 361 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac 421 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 481 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 541 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 601 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 661 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 721 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 781 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 841 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 901 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 961 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 1021 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 1081 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 1141 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 1201 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 1261 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 1321 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 1381 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 1441 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 1501 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 1561 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 1621 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 1681 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 1741 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 1801 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 1861 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 1921 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 1981 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 2041 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 2101 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 2161 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 2221 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 2281 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt 2341 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 2401 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 2461 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 2521 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 2581 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 2641 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 2701 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 2761 aacaaaatat taacgcttac aatttgccat tcgccattca ggctgcgcaa ctgttgggaa 2821 gggcgatcgg tgcgggcctc ttcgctatta cgccagccca agctaccatg ataagtaagt 2881 aatattaagg tacgggaggt acttggagcg gccgcaataa aatatcttta ttttcattac 2941 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca 3001 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa 3061 catttctcta tcgataggta ccacaagttt gtacaaaaaa gcaggcttcg ttttcacgga 3121 gtactgtcct ccgagtgtca acactctgta atccggagta ctgtcctccg agaaattaaa 3181 acaccaaatt gaatttgtgg aaggcctagg ccactggtta gaacaattga atatggagac 3241 ggaaaaatgc atgaggcatc atcctaaatt gactaccaat tttatataat gagatgggcg 3301 acactcattc cagcagatag cgaataccaa cggatgcaat tgctcaagtc gggacaatta 3361 ctgaatccga ccggagtact gtcctccgag agagatttat cagcatctcg aaaattacac 3421 tcgatgtaca caccaattgc tcaattggga gtctttagtc attccgcact cgacagataa 3481 gctttcgcta ctgctgctaa taaaatttcg ccaataatgc aaatcagatc ttatcgcagg 3541 ccaactggga cccagctttc ttgtacaaag tggtagatct gcgatcgagc tcgcccgggg 3601 atcgagcgca gcggtataaa agggcgcggg gtggctgaga gcatcagttg tgaatgaatg 3661 ttcgagccga gcagacgtgc cgctgccttc gttaatatcc tttgaataag ccaactttga 3721 atcacaagac gcataccaaa cggcattccg gtactgttgg taaagccacc atggaagacg 3781 ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga accgctggag 3841 agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag 3901 atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc gttcggttgg 3961 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa 4021 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc 4081 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt tcgcagccta 4141 ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc 4201 caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga 4261 tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtgccag 4321 agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga tctactggtc 4381 tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg catgccagag 4441 atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc 4501 atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct 4561 taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac aagattcaaa 4621 gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg attgacaaat 4681 acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct aaggaagtcg 4741 gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat gggctcactg 4801 agactacatc agctattctg attacacccg agggggatga taaaccgggc gcggtcggta 4861 aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg 4921 ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca 4981 atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag 5041 cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct ctgattaagt 5101 acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa caccccaaca 5161 tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg 5221 ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca 5281 gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga 5341 aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga 5401 agggcggaaa gatcgccgtg taatt //