LOCUS       Exported                3854 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pJA304
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Zeiser E, Frokjaer-Jensen C, Jorgensen E, Ahringer J
  TITLE     MosSCI and gateway compatible plasmid toolkit for constitutive and 
            inducible expression of transgenes in the C. elegans germline.
  JOURNAL   PLoS One. 2011;6(5):e20082. Epub 2011 May 26.
  PUBMED    21637852
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(624..748)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      1636..1959
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(1963..2058)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2095..2114)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2096..2114)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2119..2135)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2119..2135)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2237..2256)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2248..3057
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2315..2334)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3204..3792
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3693..3712
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
      601 taataccatc taagtagttg attcatagtg actgcatatg ttgtgtttta cagtattatg
      661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      721 ctcgttcaac tttcttgtac aaagtggagg gaggtggagg tggagctatg gtctcaaagg
      781 gtgaagaaga taacatggca attattaaag agtttatgcg tttcaaggtg catatggagg
      841 gatctgtcaa tgggcatgag tttgaaattg aaggtgaagg agaaggccga ccatatgagg
      901 gaacacaaac cgcaaaacta aaggtaagtt taaacatata tatactaact aaccctgatt
      961 atttaaattt tcaggtaact aaaggcggac cattaccatt cgcctgggac atcctctctc
     1021 cacagttcat gtatggaagt aaagcttatg ttaaacatcc ggcagatata ccagattatt
     1081 tgaaactttc attcccggag ggttttaagt gggaacgcgt aatgaatttt gaagacggag
     1141 gagttgttac agtgacgcaa gactcaaggt aagtttaaac agttcggtac taactaacca
     1201 tacatattta aattttcagc ctccaagatg gagaatttat ttataaagtc aaacttcgag
     1261 gaacgaattt cccctcggat ggacctgtta tgcagaagaa gactatggga tgggaagctt
     1321 caagtgaaag aatgtaccct gaagacggtg ctcttaaggg agagattaaa caacgtctta
     1381 aattgaaaga tggaggacat tacgatgctg aggtaagttt aaacatgatt ttactaacta
     1441 actaatctga tttaaatttt caggtgaaga caacttacaa agccaaaaaa ccagttcagc
     1501 tgccaggagc gtacaatgtt aatattaaac tggatatcac ctcccacaac gaggattaca
     1561 ctatcgttga gcaatatgaa agagctgaag ggcggcactc gacaggtggc atggatgaat
     1621 tgtataagta aataaatgca aaatcctttc aagcattccc ttcttctcta tcactcttct
     1681 ttctttttgt caaaaaattc tctcgctaat ttatttgctt ttttaatgtt attattttat
     1741 gactttttat agtcactgaa aagtttgcat ctgagtgaag tgaatgctat caaaatgtga
     1801 ttctgtctga tgtactttca caatctctct tcaattccat tttgaagtgc tttaaacccg
     1861 aaaggttgag aaaaatgcga gcgctcaaat atttgtattg tgttcgttga gtgacccaac
     1921 aaaaagagga aactttattg tgccgccaag aaaaaagtct cacaacttta ttatacaaag
     1981 ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     2041 aaaataaaat cattatttgg agctccatgg tagcgttaac gcggccgcga tatcccctat
     2101 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
     2161 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
     2221 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
     2281 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     2341 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     2401 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     2461 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     2521 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     2581 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     2641 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     2701 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     2761 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     2821 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     2881 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     2941 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     3001 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     3061 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     3121 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     3181 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3241 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3301 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     3361 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3421 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3481 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3541 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
     3601 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3661 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3721 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3781 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3841 tttgctcaca tgtt
//
