LOCUS Exported 4962 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS TRX1 flag SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4962) AUTHORS Liu Y, Min W TITLE Thioredoxin promotes ASK1 ubiquitination and degradation to inhibit ASK1-mediated apoptosis in a redox activity-independent manner. JOURNAL Circ Res. 2002 Jun 28. 90(12):1259-66. PUBMED 12089063 REFERENCE 2 (bases 1 to 4962) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4962 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(11..28) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(182..770) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(262..281) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(941..1801) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1564..1583 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1802..1906) /gene="bla" /label=AmpR promoter rep_origin 1933..2388 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2020..2039) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2230..2251 /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2514..2536 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 2528..2545 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 2529..2545 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" enhancer 2706..3085 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 3086..3289 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 3240..3260 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 3323..3346 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" polyA_signal 3712..4334 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3911..3930) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" primer_bind complement(4358..4378) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 4523..4657 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4584..4603 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(4709..4728) /label=T7 /note="T7 promoter, forward primer" promoter complement(4710..4728) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4742..4758) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4742..4758) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4755..4777) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4766..4782 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4790..4820) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4835..4856 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." ORIGIN 1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 1921 aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 1981 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 2041 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 2101 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 2161 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 2221 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 2281 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 2341 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc attcgccatt 2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 2461 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 2521 acgacgttgt aaaacgacgg ccagtgccaa gctgatctat acattgaatc aatattggca 2581 attagccata ttagtcattg gttatatagc ataaatcaat attggctatt ggccattgca 2641 tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc 2701 atgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca 2761 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 2821 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 2881 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 2941 acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc 3001 cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta 3061 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca atgggcgtgg 3121 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt 3181 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg ccccgttgac 3241 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa 3301 ccgtcagaat tgatctacca tggactacaa agacgatgac gacaagcttg cggccgcgaa 3361 ttctgcagtc gacggtacca tggtgaagca gatcgagagc aagactgctt ttcaggaagc 3421 cttggacgct gcaggtgata aacttgtagt agttgacttc tcagccacgt ggtgtgggcc 3481 ttgcaaaatg atcaagcctt tctttcattc cctctctgaa aagtattcca acgtgatatt 3541 ccttgaagta gatgtggatg actgtcagga tgttgcttca gagtgtgaag tcaaatgcat 3601 gccaacattc cagtttttta agaagggaca aaaggtgggt gaattttctg gagccaataa 3661 ggaaaagctt gaagccacca ttaatgaatt agtctaagtc tagaggatcc cgggtggcat 3721 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc 3781 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat 3841 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg 3901 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca 3961 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc 4021 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc 4081 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc 4141 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa 4201 taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg 4261 gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag 4321 atgcctgttg aattgggtac gcggccagct tggctgtgga atgtgtgtca gttagggtgt 4381 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 4441 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 4501 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccagttccgc ccattctccg 4561 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 4621 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcctc 4681 gaggaactga aaaaccagaa agttaattcc ctatagtgag tcgtattaaa ttcgtaatca 4741 tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 4801 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 4861 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 4921 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ct //